Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1347930..1348555 Replicon chromosome
Accession NZ_AP026907
Organism Escherichia coli strain MY732

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag MY015_RS06550 Protein ID WP_000911329.1
Coordinates 1348157..1348555 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag MY015_RS06545 Protein ID WP_000450524.1
Coordinates 1347930..1348157 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY015_RS06520 (1343732) 1343732..1344202 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
MY015_RS06525 (1344202) 1344202..1344774 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
MY015_RS06530 (1344920) 1344920..1345798 + 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
MY015_RS06535 (1345815) 1345815..1346849 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
MY015_RS06540 (1347062) 1347062..1347775 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
MY015_RS06545 (1347930) 1347930..1348157 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
MY015_RS06550 (1348157) 1348157..1348555 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
MY015_RS06555 (1348702) 1348702..1349565 + 864 WP_001267498.1 neutral zinc metallopeptidase -
MY015_RS06560 (1349580) 1349580..1351595 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
MY015_RS06565 (1351669) 1351669..1352367 + 699 WP_000679823.1 esterase -
MY015_RS06570 (1352477) 1352477..1352677 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T42813 WP_000911329.1 NZ_AP026907:1348157-1348555 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T42813 NZ_AP026907:1348157-1348555 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT42813 WP_000450524.1 NZ_AP026907:1347930-1348157 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT42813 NZ_AP026907:1347930-1348157 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References