Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 913916..914570 Replicon chromosome
Accession NZ_AP026907
Organism Escherichia coli strain MY732

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag MY015_RS04495 Protein ID WP_000244777.1
Coordinates 914163..914570 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MY015_RS04490 Protein ID WP_000354046.1
Coordinates 913916..914182 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY015_RS04465 (909085) 909085..909828 + 744 WP_000951948.1 SDR family oxidoreductase -
MY015_RS04470 (909885) 909885..911318 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
MY015_RS04475 (911363) 911363..911674 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
MY015_RS04480 (911838) 911838..912497 + 660 WP_000250274.1 hemolysin III family protein -
MY015_RS04485 (912693) 912693..913673 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MY015_RS04490 (913916) 913916..914182 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MY015_RS04495 (914163) 914163..914570 + 408 WP_000244777.1 protein YgfX Toxin
MY015_RS04500 (914610) 914610..915131 - 522 WP_001055870.1 flavodoxin FldB -
MY015_RS04505 (915243) 915243..916139 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MY015_RS04510 (916164) 916164..916874 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MY015_RS04515 (916880) 916880..918613 + 1734 WP_128995306.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T42811 WP_000244777.1 NZ_AP026907:914163-914570 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T42811 NZ_AP026907:914163-914570 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT42811 WP_000354046.1 NZ_AP026907:913916-914182 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT42811 NZ_AP026907:913916-914182 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References