Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 268782..269368 Replicon chromosome
Accession NZ_AP026907
Organism Escherichia coli strain MY732

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag MY015_RS01225 Protein ID WP_042014162.1
Coordinates 268782..269012 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag MY015_RS01230 Protein ID WP_000593555.1
Coordinates 269009..269368 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY015_RS01215 (264819) 264819..267554 + 2736 WP_000149168.1 ribosome-associated ATPase/putative transporter RbbA -
MY015_RS01220 (267554) 267554..268678 + 1125 WP_001314210.1 ABC transporter permease -
MY015_RS01225 (268782) 268782..269012 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
MY015_RS01230 (269009) 269009..269368 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MY015_RS01235 (269488) 269488..269889 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
MY015_RS01240 (269895) 269895..270701 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
MY015_RS01245 (270698) 270698..271462 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
MY015_RS01250 (271462) 271462..272295 - 834 WP_001008968.1 nickel ABC transporter permease subunit NikC -
MY015_RS01255 (272292) 272292..273236 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T42807 WP_042014162.1 NZ_AP026907:268782-269012 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T42807 NZ_AP026907:268782-269012 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT42807 WP_000593555.1 NZ_AP026907:269009-269368 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT42807 NZ_AP026907:269009-269368 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References