Detailed information of TA system TA042804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4819371..4819987
• Replicon: chromosome
• Accession: NZ_AP026886
• Organism: Enterobacter asburiae strain KAM576

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: OO861_RS23485
• Protein ID: WP_083239553.1
• Coordinates: 4819371..4819742 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: OO861_RS23490
• Protein ID: WP_023309753.1
• Coordinates: 4819745..4819987 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO861_RS23470 (KAM576c_45850)	4816871..4817773	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
OO861_RS23475 (KAM576c_45860)	4817770..4818405	+	636	WP_008501832.1	formate dehydrogenase cytochrome b556 subunit	-
OO861_RS23480 (KAM576c_45870)	4818402..4819331	+	930	WP_023309751.1	formate dehydrogenase accessory protein FdhE	-
OO861_RS23485 (KAM576c_45880)	4819371..4819742	-	372	WP_083239553.1	type II toxin-antitoxin system VapC family toxin	Toxin
OO861_RS23490 (KAM576c_45890)	4819745..4819987	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
OO861_RS23495 (KAM576c_45900)	4820187..4821107	+	921	WP_069303436.1	alpha/beta hydrolase	-
OO861_RS23500 (KAM576c_45910)	4821116..4822057	-	942	WP_193140221.1	fatty acid biosynthesis protein FabY	-
OO861_RS23505 (KAM576c_45920)	4822102..4822539	-	438	WP_008501836.1	D-aminoacyl-tRNA deacylase	-
OO861_RS23510 (KAM576c_45930)	4822536..4823417	-	882	WP_033146965.1	virulence factor BrkB family protein	-
OO861_RS23515 (KAM576c_45940)	4823411..4824010	-	600	WP_064673051.1	glucose-1-phosphatase	-
OO861_RS23520 (KAM576c_45950)	4824099..4824569	-	471	WP_033146967.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13716.86 Da
• Isoelectric Point: 6.9790

>T42804 WP_083239553.1 NZ_AP026886:c4819742-4819371 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMRLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T42804 NZ_AP026886:c4819742-4819371 [Enterobacter asburiae]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCGCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTCTGCGC
GAGAAGCATGGAATGAGACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAATTGTCCGTTGATTTCTCGAAA
CACGAAAGACTTTTCGGGCATTACTGGAGTCGTTTCACCTTACCAACTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9429.56 Da
• Isoelectric Point: 4.2803

>AT42804 WP_023309753.1 NZ_AP026886:c4819987-4819745 [Enterobacter asburiae]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT42804 NZ_AP026886:c4819987-4819745 [Enterobacter asburiae]
ATGAGTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGATGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTTGACCAGCAAAGTTCATCGG
TTATCCGCGATGCGCTTGGTTTGTGGGGAAATCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0