Detailed information of TA system TA042800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3196325..3196915
• Replicon: chromosome
• Accession: NZ_AP026886
• Organism: Enterobacter asburiae strain KAM576

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: OO861_RS15750
• Protein ID: WP_064672767.1
• Coordinates: 3196325..3196657 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: OO861_RS15755
• Protein ID: WP_033145928.1
• Coordinates: 3196658..3196915 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO861_RS15725 (KAM576c_30870)	3191884..3192702	-	819	WP_032454852.1	hypothetical protein	-
OO861_RS15730 (KAM576c_30880)	3192699..3193892	-	1194	WP_094948193.1	toll/interleukin-1 receptor domain-containing protein	-
OO861_RS15735 (KAM576c_30890)	3193894..3194406	-	513	WP_081178146.1	TIR domain-containing protein	-
OO861_RS15740 (KAM576c_30900)	3194448..3195059	-	612	WP_081178148.1	TIR domain-containing protein	-
OO861_RS15745 (KAM576c_30910)	3195059..3195787	-	729	WP_032454839.1	hypothetical protein	-
OO861_RS15750 (KAM576c_30920)	3196325..3196657	-	333	WP_064672767.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OO861_RS15755 (KAM576c_30930)	3196658..3196915	-	258	WP_033145928.1	antitoxin	Antitoxin
OO861_RS15760 (KAM576c_30940)	3197207..3197596	-	390	WP_059445031.1	RidA family protein	-
OO861_RS15765	3197738..3198657	+	920	Protein_3093	LysR family transcriptional regulator	-
OO861_RS15770 (KAM576c_30970)	3198732..3199034	-	303	WP_058841454.1	XRE family transcriptional regulator	-
OO861_RS15775 (KAM576c_30980)	3199039..3199392	-	354	WP_193139527.1	type II toxin-antitoxin system RelE/ParE family toxin	-
OO861_RS15780 (KAM576c_30990)	3199570..3200463	-	894	WP_193139529.1	LysR substrate-binding domain-containing protein	-
OO861_RS15785 (KAM576c_31000)	3200634..3201242	+	609	WP_143346794.1	glutathione S-transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11798.63 Da
• Isoelectric Point: 9.4051

>T42800 WP_064672767.1 NZ_AP026886:c3196657-3196325 [Enterobacter asburiae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEGAGTTTTGVIRCDQPRTI
DMAARNGMRLERIPDAVVNEVLARLDAILN

Nucleotide

• Length: 333 bp

>T42800 NZ_AP026886:c3196657-3196325 [Enterobacter asburiae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCGCTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTTCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTAGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCGGGAACAACAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCATGCGGCTGGAACGCATACCTGACGCCGTTGTAAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTGAATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9464.92 Da
• Isoelectric Point: 4.4737

>AT42800 WP_033145928.1 NZ_AP026886:c3196915-3196658 [Enterobacter asburiae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEEREWIDAPAV
GREIL

Nucleotide

• Length: 258 bp

>AT42800 NZ_AP026886:c3196915-3196658 [Enterobacter asburiae]
ATGTATACAACTCGTCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAGCGGCCTCACTATTCGC
TTGAGGAGTTGCTGGCGCAGTGCGATCCGCACGCTGAAATGAGCGAAGAGGAGCGGGAATGGATCGATGCGCCTGCGGTG
GGCAGGGAGATCCTGTAA