Detailed information of TA system TA042790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 585590..586166
• Replicon: chromosome
• Accession: NZ_AP026886
• Organism: Enterobacter asburiae strain KAM576

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: OO861_RS02750
• Protein ID: WP_193138830.1
• Coordinates: 585590..585877 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A411GC49
• Locus tag: OO861_RS02755
• Protein ID: WP_033144548.1
• Coordinates: 585864..586166 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO861_RS02725 (KAM576c_05290)	580707..581402	+	696	WP_193138828.1	winged helix-turn-helix domain-containing protein	-
OO861_RS02730 (KAM576c_05300)	581551..582021	+	471	WP_064673177.1	MarR family transcriptional regulator	-
OO861_RS02735 (KAM576c_05310)	582018..583085	+	1068	WP_109846354.1	HlyD family secretion protein	-
OO861_RS02740 (KAM576c_05320)	583075..584151	+	1077	WP_182383002.1	DUF2955 domain-containing protein	-
OO861_RS02745 (KAM576c_05330)	584148..585419	-	1272	WP_193138829.1	DUF445 domain-containing protein	-
OO861_RS02750 (KAM576c_05340)	585590..585877	+	288	WP_193138830.1	BrnT family toxin	Toxin
OO861_RS02755 (KAM576c_05350)	585864..586166	+	303	WP_033144548.1	BrnA antitoxin family protein	Antitoxin
OO861_RS02760 (KAM576c_05360)	586197..586835	-	639	WP_193138831.1	LysE family translocator	-
OO861_RS02765 (KAM576c_05370)	586883..587572	-	690	WP_226852701.1	AraC family transcriptional regulator	-
OO861_RS02770 (KAM576c_05390)	587778..589148	+	1371	WP_193138832.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
OO861_RS02775 (KAM576c_05400)	589194..589514	-	321	WP_182383010.1	hypothetical protein	-
OO861_RS02780 (KAM576c_05410)	589514..590059	-	546	WP_023310269.1	YfaZ family outer membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11188.66 Da
• Isoelectric Point: 6.7609

>T42790 WP_193138830.1 NZ_AP026886:585590-585877 [Enterobacter asburiae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTIRFESGDEVIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T42790 NZ_AP026886:585590-585877 [Enterobacter asburiae]
ATGCCAACGGAGTTTGAATGGGATACCAACAAAGCGAAAAGCAATCTCATTAAGCATGGGATCCGCTTTGAAGAAGCTGT
TCTGGTGTTTGACGATCCTTACCATTTATCTTTGCAGGATCGTCATGAAAATGGAGAGTTCCGTTGGCAGACCATTGGCC
TTGTACATGGTCTAATTGTCATTATGGTCGCACACACGATACGTTTTGAAAGTGGTGACGAAGTCATTCGAATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAGCCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11395.04 Da
• Isoelectric Point: 10.2483

>AT42790 WP_033144548.1 NZ_AP026886:585864-586166 [Enterobacter asburiae]
MSMVKHKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT42790 NZ_AP026886:585864-586166 [Enterobacter asburiae]
ATGAGCATGGTTAAACATAAACGCGGTAGTGCGCCTGAGATGAGTGCTCGCCGTGAGGCTGAGCTCAAAGCACTAGCCGA
AAAATCGGAAGCAGAGATTGATTACAGCGATATCCCTGCCACGGGAGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTCAAAACTCAGGCGTCTGTACGTATCGACGCTGACGTGATGGAGTGGCTTAAACGGCCTGGAAAAGGT
TACCAGACGAGGCTCAATGCCATTTTGCGTGAAGCTATGCTGCGCGATCAAAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A411GC49