Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 259821..260417 Replicon chromosome
Accession NZ_AP026886
Organism Enterobacter asburiae strain KAM576

Toxin (Protein)


Gene name PumA Uniprot ID A0A4R0G5K0
Locus tag OO861_RS01165 Protein ID WP_039264167.1
Coordinates 260115..260417 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A808IFF3
Locus tag OO861_RS01160 Protein ID WP_033144375.1
Coordinates 259821..260108 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO861_RS01155 (KAM576c_02220) 258193..259824 + 1632 WP_033144374.1 Na/Pi cotransporter family protein -
OO861_RS01160 (KAM576c_02230) 259821..260108 - 288 WP_033144375.1 putative addiction module antidote protein Antitoxin
OO861_RS01165 (KAM576c_02240) 260115..260417 - 303 WP_039264167.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OO861_RS01170 (KAM576c_02250) 260615..261487 + 873 WP_033144377.1 23S rRNA pseudouridine(2604) synthase RluF -
OO861_RS01175 (KAM576c_02260) 261488..261760 - 273 WP_023309994.1 DUF3811 domain-containing protein -
OO861_RS01180 (KAM576c_02270) 261811..262755 - 945 WP_029740629.1 ketopantoate/pantoate/pantothenate transporter PanS -
OO861_RS01185 (KAM576c_02280) 262849..264198 - 1350 WP_023309996.1 lysine-sensitive aspartokinase 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-93)

Antitoxin

Uncharacterized protein HI1420

(3-89)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11401.17 Da        Isoelectric Point: 9.6038

>T42789 WP_039264167.1 NZ_AP026886:c260417-260115 [Enterobacter asburiae]
MKEIVQTESFQRWEQNLKDRRAKTIIASRLFRLANGLAGDIKPVGEGISELRIHYGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Download         Length: 303 bp

>T42789 NZ_AP026886:c260417-260115 [Enterobacter asburiae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCAACGCTGGGAGCAAAACTTAAAAGATCGGCGAGCGAAAACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGTTTAGCGGGCGACATTAAACCCGTGGGCGAAGGTATAAGTGAGCTGAGGATCC
ACTATGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10111.59 Da        Isoelectric Point: 6.4596

>AT42789 WP_033144375.1 NZ_AP026886:c260108-259821 [Enterobacter asburiae]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKHAGD

Download         Length: 288 bp

>AT42789 NZ_AP026886:c260108-259821 [Enterobacter asburiae]
ATGCATAAATTAACACCCTACGATCCAGCCAATGCGCTGGTGGATGACGAGGAAATCGCCGTGTTTATGGCTGATGCCTT
AGAAACGGGCGACTCAGCGTATATTGCTAAAGCTCTGGGCGTGATCGCGCGAGCGAAAGGAATGTCGACCATTTCACAGC
AAACTGGCCTATCACGAGAGCAACTGTATCGATCGTTCAGTGATAAAGGAAACCCAACGCTTAAAACCACTCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTAACTATCAAACACGCCGGAGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0G5K0

Antitoxin

Source ID Structure
AlphaFold DB A0A808IFF3

References