Detailed information of TA system TA042787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 55755..56417
• Replicon: chromosome
• Accession: NZ_AP026886
• Organism: Enterobacter asburiae strain KAM576

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: OO861_RS00275
• Protein ID: WP_176686521.1
• Coordinates: 56019..56417 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7W3DB74
• Locus tag: OO861_RS00270
• Protein ID: WP_039260382.1
• Coordinates: 55755..56015 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO861_RS00255 (KAM576c_00480)	53180..54301	-	1122	WP_182382842.1	efflux RND transporter periplasmic adaptor subunit	-
OO861_RS00260 (KAM576c_00490)	54450..55019	-	570	WP_033144322.1	helix-turn-helix domain-containing protein	-
OO861_RS00265 (KAM576c_00500)	55206..55625	+	420	WP_039260381.1	GNAT family N-acetyltransferase	-
OO861_RS00270 (KAM576c_00510)	55755..56015	+	261	WP_039260382.1	virulence protein	Antitoxin
OO861_RS00275 (KAM576c_00520)	56019..56417	+	399	WP_176686521.1	type II toxin-antitoxin system VapC family toxin	Toxin
OO861_RS00280 (KAM576c_00530)	56558..57358	+	801	WP_182382841.1	lipoprotein NlpA	-
OO861_RS00285 (KAM576c_00540)	57481..58260	+	780	WP_182382840.1	MBL fold metallo-hydrolase	-
OO861_RS00290 (KAM576c_00550)	58287..59240	+	954	WP_048981419.1	helix-turn-helix domain-containing protein	-
OO861_RS00295 (KAM576c_00560)	59424..61121	+	1698	WP_226852994.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14961.16 Da
• Isoelectric Point: 6.0840

>T42787 WP_176686521.1 NZ_AP026886:56019-56417 [Enterobacter asburiae]
MLHMLDTNMVSHLVRQHPGVLEQYSRTPTEKMCISSVTEAELLYGIAKKQNDTLQKTILEFLKTITICDWDSDAAATYGE
LRAAMEKRGKVMGDLDQLIAAHAISRRTTIVTNDRAFRMVQELTVEDWTTTS

Nucleotide

• Length: 399 bp

>T42787 NZ_AP026886:56019-56417 [Enterobacter asburiae]
ATGCTGCATATGTTGGATACAAACATGGTAAGCCATCTCGTGAGACAACATCCTGGGGTGTTGGAACAATATTCTCGCAC
CCCAACTGAAAAAATGTGTATTTCAAGCGTAACGGAGGCTGAATTGCTTTATGGGATAGCCAAAAAGCAAAACGACACAT
TACAAAAAACCATACTGGAATTTCTTAAAACCATTACGATTTGCGACTGGGATAGTGACGCCGCCGCAACGTACGGCGAA
CTCCGCGCAGCAATGGAGAAGAGAGGAAAAGTGATGGGCGATCTCGATCAACTGATCGCTGCGCATGCTATCAGTCGGAG
AACAACGATTGTGACTAACGATCGTGCATTCAGAATGGTGCAGGAGCTTACCGTCGAGGACTGGACGACAACGTCGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10176.44 Da
• Isoelectric Point: 8.5750

>AT42787 WP_039260382.1 NZ_AP026886:55755-56015 [Enterobacter asburiae]
MERTAKLFKKGRNQAVMLPAEFAFDTESVYIRRDEEGNVVLTARSEKERHRDNFLRLLKQTHVPDSFLSKEERNQSYTTR
DPFEGL

Nucleotide

• Length: 261 bp

>AT42787 NZ_AP026886:55755-56015 [Enterobacter asburiae]
ATGGAACGCACAGCAAAACTCTTCAAAAAGGGGCGGAATCAGGCCGTAATGCTACCTGCTGAATTCGCGTTTGATACGGA
GAGCGTCTACATCCGGCGGGATGAGGAGGGGAATGTCGTTTTGACTGCGAGATCGGAGAAAGAGCGGCATCGAGATAACT
TTTTACGTTTATTGAAGCAGACCCATGTTCCTGATTCATTCCTCAGCAAAGAGGAACGTAATCAGAGCTATACAACAAGA
GATCCCTTTGAAGGACTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7W3DB74