Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 93530..94173 Replicon plasmid pKAM546_1
Accession NZ_AP026874
Organism Enterobacter roggenkampii strain KAM546

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag OO938_RS23005 Protein ID WP_000754567.1
Coordinates 93530..93946 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag OO938_RS23010 Protein ID WP_023293777.1
Coordinates 93943..94173 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO938_RS22975 (KAM546c_45370) 88717..89298 + 582 WP_008502230.1 TetR/AcrR family transcriptional regulator -
OO938_RS22980 (KAM546c_45380) 89303..89641 + 339 WP_000888080.1 carboxymuconolactone decarboxylase family protein -
OO938_RS22985 (KAM546c_45390) 89671..90000 - 330 WP_202324598.1 thioredoxin family protein -
OO938_RS22990 (KAM546c_45400) 90213..91319 + 1107 WP_264884022.1 alkene reductase -
OO938_RS22995 (KAM546c_45410) 91385..92086 + 702 WP_008502228.1 DsbA family oxidoreductase -
OO938_RS23000 (KAM546c_45420) 92212..93179 + 968 Protein_96 IS5-like element IS903B family transposase -
OO938_RS23005 (KAM546c_45430) 93530..93946 - 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
OO938_RS23010 (KAM546c_45440) 93943..94173 - 231 WP_023293777.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
OO938_RS23015 (KAM546c_45450) 94836..95042 + 207 WP_004153649.1 hypothetical protein -
OO938_RS23020 (KAM546c_45460) 95088..95396 + 309 WP_004152336.1 hypothetical protein -
OO938_RS23025 (KAM546c_45470) 95424..95753 + 330 WP_004152337.1 hypothetical protein -
OO938_RS23030 (KAM546c_45480) 95779..96177 + 399 WP_004171440.1 hypothetical protein -
OO938_RS23035 (KAM546c_45490) 96184..96516 + 333 WP_004152339.1 hypothetical protein -
OO938_RS23040 (KAM546c_45500) 96516..97298 + 783 WP_004152340.1 site-specific integrase -
OO938_RS23045 (KAM546c_45510) 98190..98420 - 231 WP_011977773.1 hypothetical protein -
OO938_RS23050 (KAM546c_45520) 98512..98850 - 339 WP_004198970.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-10 mrkJ / mrkF / mrkC / mrkB / mrkA 1..139510 139510


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T42786 WP_000754567.1 NZ_AP026874:c93946-93530 [Enterobacter roggenkampii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T42786 NZ_AP026874:c93946-93530 [Enterobacter roggenkampii]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT42786 WP_023293777.1 NZ_AP026874:c94173-93943 [Enterobacter roggenkampii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT42786 NZ_AP026874:c94173-93943 [Enterobacter roggenkampii]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGCAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGATCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References