Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 3892532..3893211 Replicon chromosome
Accession NZ_AP026873
Organism Enterobacter roggenkampii strain KAM546

Toxin (Protein)


Gene name ykfI Uniprot ID A0A7D6Z078
Locus tag OO938_RS19190 Protein ID WP_048215514.1
Coordinates 3892532..3892873 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0F6RHY7
Locus tag OO938_RS19195 Protein ID WP_046495841.1
Coordinates 3892894..3893211 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO938_RS19170 (KAM546c_38000) 3888836..3890683 + 1848 WP_008503184.1 RNA polymerase sigma factor RpoD -
OO938_RS19175 (KAM546c_38010) 3890774..3891280 - 507 WP_008503183.1 G/U mismatch-specific DNA glycosylase -
OO938_RS19185 (KAM546c_38020) 3891583..3892416 - 834 WP_046495848.1 DUF4942 domain-containing protein -
OO938_RS19190 (KAM546c_38030) 3892532..3892873 - 342 WP_048215514.1 TA system toxin CbtA family protein Toxin
OO938_RS19195 (KAM546c_38040) 3892894..3893211 - 318 WP_046495841.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
OO938_RS19200 (KAM546c_38050) 3893230..3893451 - 222 WP_000691995.1 DUF987 domain-containing protein -
OO938_RS19205 (KAM546c_38060) 3893460..3893936 - 477 WP_264881436.1 RadC family protein -
OO938_RS19210 (KAM546c_38070) 3893952..3894410 - 459 WP_039026309.1 antirestriction protein -
OO938_RS19215 (KAM546c_38080) 3894525..3894749 - 225 WP_039026310.1 DUF905 domain-containing protein -
OO938_RS19220 (KAM546c_38090) 3894845..3895780 - 936 WP_072038556.1 Ivy family c-type lysozyme inhibitor -
OO938_RS19225 (KAM546c_38100) 3895831..3896046 - 216 WP_039026311.1 hypothetical protein -
OO938_RS19230 (KAM546c_38110) 3896374..3897072 - 699 WP_039026312.1 DeoR family transcriptional regulator -
OO938_RS19235 (KAM546c_38120) 3897281..3898105 - 825 WP_264881440.1 DUF932 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - hcp/tssD 3890774..3920732 29958


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(2-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13014.97 Da        Isoelectric Point: 10.4182

>T42784 WP_048215514.1 NZ_AP026873:c3892873-3892532 [Enterobacter roggenkampii]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRRRF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T42784 NZ_AP026873:c3892873-3892532 [Enterobacter roggenkampii]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGTCACCCGTGGCTATCTGGCAAATTCTACTGAC
ACGCCTGCTGGAACAACACTACGGCCTGACCCTGAACGACACGCCGTTCAGGGATGAATCCGTTATACAGGAACATATCG
ATGCAGGTATCACGCTGGCCAATGCCGTTAACTTTCTGGTGGAAAAATACGAGCTTGTTCGTATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCAGCAGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11869.49 Da        Isoelectric Point: 6.6245

>AT42784 WP_046495841.1 NZ_AP026873:c3893211-3892894 [Enterobacter roggenkampii]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Download         Length: 318 bp

>AT42784 NZ_AP026873:c3893211-3892894 [Enterobacter roggenkampii]
ATGAGCAACATCACTTGGGGCTTGCAGCGCGATATCACGCCGCGCCTGGGAACACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTACCTAGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCTCCGGTGAACTGAATCCCCTACACGTTCATTGCGTCACCCTGTACCAC
ACCGGTTTTACCTGCGAAGCCGACACCCTCGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTAAGCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6Z078

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6RHY7

References