Detailed information of TA system TA042783

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 3789707..3790500
• Replicon: chromosome
• Accession: NZ_AP026873
• Organism: Enterobacter roggenkampii strain KAM546

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: OO938_RS18705
• Protein ID: WP_000854726.1
• Coordinates: 3790123..3790500 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: OO938_RS18700
• Protein ID: WP_001548930.1
• Coordinates: 3789707..3790033 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO938_RS18665 (KAM546c_36950)	3785377..3786270	+	894	WP_032666736.1	50S ribosome-binding GTPase	-
OO938_RS18670 (KAM546c_36960)	3786560..3787237	+	678	WP_137376005.1	hypothetical protein	-
OO938_RS18675 (KAM546c_36970)	3787339..3788160	+	822	WP_167819612.1	DUF932 domain-containing protein	-
OO938_RS18680 (KAM546c_36980)	3788168..3788422	+	255	WP_050482906.1	DUF905 domain-containing protein	-
OO938_RS18685 (KAM546c_36990)	3788496..3788966	+	471	WP_182945219.1	antirestriction protein	-
OO938_RS18690 (KAM546c_37000)	3788982..3789458	+	477	WP_000811693.1	RadC family protein	-
OO938_RS18695 (KAM546c_37010)	3789467..3789688	+	222	WP_000691994.1	DUF987 domain-containing protein	-
OO938_RS18700 (KAM546c_37020)	3789707..3790033	+	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
OO938_RS18705 (KAM546c_37030)	3790123..3790500	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
OO938_RS18710 (KAM546c_37040)	3790497..3790985	+	489	WP_001547769.1	DUF5983 family protein	-
OO938_RS18715 (KAM546c_37050)	3790997..3791194	+	198	WP_000839269.1	DUF957 domain-containing protein	-
OO938_RS18720 (KAM546c_37060)	3791279..3792121	+	843	WP_182945218.1	DUF4942 domain-containing protein	-
OO938_RS18725 (KAM546c_37070)	3792309..3792836	-	528	WP_052259168.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T42783 WP_000854726.1 NZ_AP026873:3790123-3790500 [Enterobacter roggenkampii]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T42783 NZ_AP026873:3790123-3790500 [Enterobacter roggenkampii]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT42783 WP_001548930.1 NZ_AP026873:3789707-3790033 [Enterobacter roggenkampii]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT42783 NZ_AP026873:3789707-3790033 [Enterobacter roggenkampii]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7