Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1073518..1074138 Replicon chromosome
Accession NZ_AP026873
Organism Enterobacter roggenkampii strain KAM546

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag OO938_RS05320 Protein ID WP_008499287.1
Coordinates 1073518..1073736 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag OO938_RS05325 Protein ID WP_008499288.1
Coordinates 1073764..1074138 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO938_RS05290 (KAM546c_10450) 1069531..1069791 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
OO938_RS05295 1069794..1069934 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
OO938_RS05300 (KAM546c_10460) 1069931..1070641 - 711 WP_264882619.1 GNAT family protein -
OO938_RS05305 (KAM546c_10470) 1070743..1072203 + 1461 WP_264882621.1 PLP-dependent aminotransferase family protein -
OO938_RS05310 (KAM546c_10480) 1072175..1072642 - 468 WP_008499285.1 YlaC family protein -
OO938_RS05315 (KAM546c_10490) 1072760..1073311 - 552 WP_264882623.1 maltose O-acetyltransferase -
OO938_RS05320 (KAM546c_10500) 1073518..1073736 - 219 WP_008499287.1 HHA domain-containing protein Toxin
OO938_RS05325 (KAM546c_10510) 1073764..1074138 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
OO938_RS05330 (KAM546c_10520) 1074543..1075523 - 981 WP_044713288.1 IS5-like element IS5 family transposase -
OO938_RS05335 (KAM546c_10530) 1075847..1078993 - 3147 WP_264882625.1 multidrug efflux RND transporter permease subunit AcrB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1074543..1075523 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T42772 WP_008499287.1 NZ_AP026873:c1073736-1073518 [Enterobacter roggenkampii]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T42772 NZ_AP026873:c1073736-1073518 [Enterobacter roggenkampii]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTCGAACGCGT
GATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCCGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAGC
TGACCATGAATAAGTTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT42772 WP_008499288.1 NZ_AP026873:c1074138-1073764 [Enterobacter roggenkampii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT42772 NZ_AP026873:c1074138-1073764 [Enterobacter roggenkampii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAACCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References