Detailed information of TA system TA042770

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 372123..372916
• Replicon: chromosome
• Accession: NZ_AP026873
• Organism: Enterobacter roggenkampii strain KAM546

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: OO938_RS01740
• Protein ID: WP_000854726.1
• Coordinates: 372123..372500 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: OO938_RS01745
• Protein ID: WP_001548930.1
• Coordinates: 372590..372916 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO938_RS01725 (KAM546c_03390)	370502..371344	-	843	WP_182945218.1	DUF4942 domain-containing protein	-
OO938_RS01730 (KAM546c_03400)	371429..371626	-	198	WP_000839269.1	DUF957 domain-containing protein	-
OO938_RS01735 (KAM546c_03410)	371638..372126	-	489	WP_001547769.1	DUF5983 family protein	-
OO938_RS01740 (KAM546c_03420)	372123..372500	-	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
OO938_RS01745 (KAM546c_03430)	372590..372916	-	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
OO938_RS01750 (KAM546c_03440)	372935..373155	-	221	Protein_336	DUF987 domain-containing protein	-
OO938_RS01755 (KAM546c_03450)	373164..373640	-	477	WP_000811693.1	RadC family protein	-
OO938_RS01760 (KAM546c_03460)	373656..374126	-	471	WP_182945219.1	antirestriction protein	-
OO938_RS01765 (KAM546c_03470)	374200..374454	-	255	WP_050482906.1	DUF905 domain-containing protein	-
OO938_RS01770 (KAM546c_03480)	374462..375283	-	822	WP_167819612.1	DUF932 domain-containing protein	-
OO938_RS01775 (KAM546c_03490)	375385..376062	-	678	WP_137376005.1	hypothetical protein	-
OO938_RS01780 (KAM546c_03500)	376352..377245	-	894	WP_032666736.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T42770 WP_000854726.1 NZ_AP026873:c372500-372123 [Enterobacter roggenkampii]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T42770 NZ_AP026873:c372500-372123 [Enterobacter roggenkampii]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT42770 WP_001548930.1 NZ_AP026873:c372916-372590 [Enterobacter roggenkampii]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT42770 NZ_AP026873:c372916-372590 [Enterobacter roggenkampii]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7