Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 34057..34634 Replicon plasmid pNUITM-VP1_1
Accession NZ_AP026828
Organism Proteus mirabilis strain NUITM-VP1

Toxin (Protein)


Gene name mazF Uniprot ID U5N651
Locus tag OO779_RS18835 Protein ID WP_023159957.1
Coordinates 34302..34634 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U5N340
Locus tag OO779_RS18830 Protein ID WP_023159984.1
Coordinates 34057..34302 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO779_RS18800 (NUITMVP1_36540) 29099..29983 + 885 WP_000155092.1 Mph(E) family macrolide 2'-phosphotransferase -
OO779_RS18805 (NUITMVP1_36550) 30067..30771 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
OO779_RS18810 (NUITMVP1_36570) 31016..31594 + 579 WP_048822182.1 hypothetical protein -
OO779_RS18815 (NUITMVP1_36590) 32355..32723 + 369 WP_048821769.1 hypothetical protein -
OO779_RS18820 (NUITMVP1_36600) 32821..33048 - 228 WP_048821767.1 plasmid partition protein ParG -
OO779_RS18825 (NUITMVP1_36610) 33157..33780 - 624 WP_023159958.1 AAA family ATPase -
OO779_RS18830 (NUITMVP1_36620) 34057..34302 + 246 WP_023159984.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
OO779_RS18835 (NUITMVP1_36630) 34302..34634 + 333 WP_023159957.1 endoribonuclease MazF Toxin
OO779_RS18840 (NUITMVP1_36640) 34665..35045 + 381 WP_048821763.1 hypothetical protein -
OO779_RS18845 35134..35274 - 141 WP_072022937.1 Hok/Gef family protein -
OO779_RS18850 (NUITMVP1_36650) 35594..36052 - 459 WP_094962568.1 hypothetical protein -
OO779_RS18855 (NUITMVP1_36670) 36444..36713 - 270 WP_048821758.1 hypothetical protein -
OO779_RS18860 (NUITMVP1_36680) 37175..37960 + 786 WP_232799287.1 site-specific integrase -
OO779_RS18865 (NUITMVP1_36690) 37976..38110 + 135 WP_256326490.1 hypothetical protein -
OO779_RS18870 (NUITMVP1_36700) 38229..39050 + 822 WP_001029679.1 TnsA endonuclease N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid lnu(G) / aadA14 / tet(X6) / floR / msr(E) / mph(E) / sul3 / ant(3'')-Ia / dfrA1 - 1..111709 111709


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12097.88 Da        Isoelectric Point: 7.0813

>T42767 WP_023159957.1 NZ_AP026828:34302-34634 [Proteus mirabilis]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Download         Length: 333 bp

>T42767 NZ_AP026828:34302-34634 [Proteus mirabilis]
ATGGTATCTCGATACGTACCTGATTCTGGCGATTTGATTTGGATTGACTTTGATCCAGTTGAAGGACATGAGCAGGGCGG
TCATCGTCCAGCAGTTGTCCTTAGCCCTTTTGCATACAATAATAAAGTAGGGCTCTTACTATGCGTACCATGTACAACTA
AAGGCAAAGGCTATCCATTTGAATGCGAACTCTCGAACGAAAGAGACAGCTACGCATTAGCGGATCAAGTTACATGTGTT
GACTGGAGAGCTCGTAAAGTTACTAAAAAGGGCAAAGTGGAGGCATCAGAACTGGCAGAGATAAAAGCTAAAGCAAAGGC
GTTAATAGGATAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8786.20 Da        Isoelectric Point: 5.0351

>AT42767 WP_023159984.1 NZ_AP026828:34057-34302 [Proteus mirabilis]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Download         Length: 246 bp

>AT42767 NZ_AP026828:34057-34302 [Proteus mirabilis]
ATGGCTCAGGTCACAGTAAAAAAATGGGGCAATAGCCCGTCTGTACGGCTTCCTGTTGCAATCATGCAAAAAGCGGCACT
TAGCGTTGATGATACGGTTGAAATAGCGGTAGAAGAAGGTCGTATCATCATTACGCCAGTTAAAGCGGTAGAATACTCAT
TAGATAATTTGTTAGCCGGTATCACACCGGAGAATATCCATGAGAAAGTAGACTTCGGAGCTCGAACAGGTAAGGAATTA
ATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P1BQ75

Antitoxin

Source ID Structure
AlphaFold DB A0A857E7M9

References