Detailed information of TA system TA042758

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 25612..26204
• Replicon: plasmid pIPCEC48_3
• Accession: NZ_AP026797
• Organism: Escherichia coli strain IPCEC48

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: OPA53_RS26930
• Protein ID: WP_000520549.1
• Coordinates: 25956..26204 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: OPA53_RS26925
• Protein ID: WP_000681613.1
• Coordinates: 25612..25959 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OPA53_RS26895 (IPCEC48_51690)	21610..21936	-	327	WP_000008647.1	TrbC/VirB2 family protein	-
OPA53_RS26900 (IPCEC48_51700)	21950..22570	-	621	WP_053911938.1	lytic transglycosylase domain-containing protein	-
OPA53_RS26905 (IPCEC48_51720)	22926..23159	-	234	WP_000121161.1	EexN family lipoprotein	-
OPA53_RS26910 (IPCEC48_51730)	23164..23430	-	267	WP_000160395.1	hypothetical protein	-
OPA53_RS26915 (IPCEC48_51740)	23507..24736	-	1230	WP_001694183.1	DNA relaxase/nickase TaxC	-
OPA53_RS26920 (IPCEC48_51750)	24748..25248	-	501	WP_001694182.1	DNA distortion polypeptide 1	-
OPA53_RS26925 (IPCEC48_51760)	25612..25959	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
OPA53_RS26930 (IPCEC48_51770)	25956..26204	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
OPA53_RS26935 (IPCEC48_51790)	26511..26816	-	306	WP_072182957.1	hypothetical protein	-
OPA53_RS26940 (IPCEC48_51800)	26884..27156	-	273	WP_001272373.1	hypothetical protein	-
OPA53_RS26945	27283..27603	-	321	WP_224693477.1	molecular chaperone DnaJ	-
OPA53_RS26950 (IPCEC48_51810)	27605..27832	-	228	WP_000801440.1	hypothetical protein	-
OPA53_RS26955 (IPCEC48_51820)	27819..28070	-	252	WP_001230707.1	hypothetical protein	-
OPA53_RS26960 (IPCEC48_51830)	28067..28609	-	543	WP_001083906.1	hypothetical protein	-
OPA53_RS26965 (IPCEC48_51840)	28714..29229	-	516	WP_001025395.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30638	30638

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T42758 WP_000520549.1 NZ_AP026797:c26204-25956 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T42758 NZ_AP026797:c26204-25956 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT42758 WP_000681613.1 NZ_AP026797:c25959-25612 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT42758 NZ_AP026797:c25959-25612 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGGAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCACTGAACGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1