Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4290340..4290916 Replicon chromosome
Accession NZ_AP026794
Organism Escherichia coli strain IPCEC48

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag OPA53_RS21125 Protein ID WP_001295743.1
Coordinates 4290629..4290916 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag OPA53_RS21120 Protein ID WP_000063148.1
Coordinates 4290340..4290642 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPA53_RS21105 (4286936) 4286936..4289086 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
OPA53_RS21110 (4289136) 4289136..4289339 + 204 WP_000467859.1 YbdD/YjiX family protein -
OPA53_RS21115 (4289350) 4289350..4290306 + 957 WP_001295745.1 GTPase -
OPA53_RS21120 (4290340) 4290340..4290642 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
OPA53_RS21125 (4290629) 4290629..4290916 - 288 WP_001295743.1 BrnT family toxin Toxin
OPA53_RS21130 (4291113) 4291113..4292279 + 1167 WP_000800831.1 restriction endonuclease -
OPA53_RS21135 (4292343) 4292343..4293962 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
OPA53_RS21140 (4293952) 4293952..4295256 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T42744 WP_001295743.1 NZ_AP026794:c4290916-4290629 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T42744 NZ_AP026794:c4290916-4290629 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT42744 WP_000063148.1 NZ_AP026794:c4290642-4290340 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT42744 NZ_AP026794:c4290642-4290340 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References