Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4180590..4181110 Replicon chromosome
Accession NZ_AP026794
Organism Escherichia coli strain IPCEC48

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag OPA53_RS20605 Protein ID WP_001160964.1
Coordinates 4180590..4180874 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag OPA53_RS20610 Protein ID WP_000125566.1
Coordinates 4180877..4181110 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPA53_RS20580 (4176235) 4176235..4177521 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
OPA53_RS20585 (4177521) 4177521..4178510 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
OPA53_RS20590 (4178507) 4178507..4179328 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
OPA53_RS20595 (4179331) 4179331..4179708 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
OPA53_RS20600 (4179715) 4179715..4180563 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
OPA53_RS20605 (4180590) 4180590..4180874 - 285 WP_001160964.1 CcdB family protein Toxin
OPA53_RS20610 (4180877) 4180877..4181110 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
OPA53_RS20615 (4181196) 4181196..4181675 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
OPA53_RS20620 (4181867) 4181867..4183729 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
OPA53_RS20625 (4183722) 4183722..4184252 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
OPA53_RS20630 (4184360) 4184360..4185691 - 1332 WP_001183209.1 MFS transporter -
OPA53_RS20635 (4185750) 4185750..4186037 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T42743 WP_001160964.1 NZ_AP026794:c4180874-4180590 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T42743 NZ_AP026794:c4180874-4180590 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT42743 WP_000125566.1 NZ_AP026794:c4181110-4180877 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT42743 NZ_AP026794:c4181110-4180877 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References