Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3729679..3730297 Replicon chromosome
Accession NZ_AP026794
Organism Escherichia coli strain IPCEC48

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag OPA53_RS18455 Protein ID WP_001291435.1
Coordinates 3730079..3730297 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag OPA53_RS18450 Protein ID WP_000344800.1
Coordinates 3729679..3730053 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPA53_RS18440 (3724768) 3724768..3725961 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
OPA53_RS18445 (3725984) 3725984..3729133 + 3150 WP_001132478.1 efflux RND transporter permease AcrB -
OPA53_RS18450 (3729679) 3729679..3730053 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
OPA53_RS18455 (3730079) 3730079..3730297 + 219 WP_001291435.1 HHA domain-containing protein Toxin
OPA53_RS18460 (3730471) 3730471..3731022 + 552 WP_000102539.1 maltose O-acetyltransferase -
OPA53_RS18465 (3731138) 3731138..3731608 + 471 WP_000136192.1 YlaC family protein -
OPA53_RS18470 (3731772) 3731772..3733322 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
OPA53_RS18475 (3733364) 3733364..3733717 - 354 WP_000878135.1 DUF1428 family protein -
OPA53_RS18485 (3734096) 3734096..3734407 + 312 WP_000409908.1 MGMT family protein -
OPA53_RS18490 (3734438) 3734438..3735010 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42741 WP_001291435.1 NZ_AP026794:3730079-3730297 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T42741 NZ_AP026794:3730079-3730297 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT42741 WP_000344800.1 NZ_AP026794:3729679-3730053 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT42741 NZ_AP026794:3729679-3730053 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References