Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4285590..4286166 Replicon chromosome
Accession NZ_AP026788
Organism Escherichia coli strain IPCEC42

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag OPA56_RS21125 Protein ID WP_001295743.1
Coordinates 4285879..4286166 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag OPA56_RS21120 Protein ID WP_000063148.1
Coordinates 4285590..4285892 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPA56_RS21105 (4282186) 4282186..4284336 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
OPA56_RS21110 (4284386) 4284386..4284589 + 204 WP_000467859.1 YbdD/YjiX family protein -
OPA56_RS21115 (4284600) 4284600..4285556 + 957 WP_001295745.1 GTPase -
OPA56_RS21120 (4285590) 4285590..4285892 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
OPA56_RS21125 (4285879) 4285879..4286166 - 288 WP_001295743.1 BrnT family toxin Toxin
OPA56_RS21130 (4286363) 4286363..4287529 + 1167 WP_000800831.1 restriction endonuclease -
OPA56_RS21135 (4287593) 4287593..4289212 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
OPA56_RS21140 (4289202) 4289202..4290506 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T42708 WP_001295743.1 NZ_AP026788:c4286166-4285879 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T42708 NZ_AP026788:c4286166-4285879 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT42708 WP_000063148.1 NZ_AP026788:c4285892-4285590 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT42708 NZ_AP026788:c4285892-4285590 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References