Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4175840..4176360 Replicon chromosome
Accession NZ_AP026788
Organism Escherichia coli strain IPCEC42

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag OPA56_RS20600 Protein ID WP_001160964.1
Coordinates 4175840..4176124 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag OPA56_RS20605 Protein ID WP_000125566.1
Coordinates 4176127..4176360 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPA56_RS20575 (4171485) 4171485..4172771 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
OPA56_RS20580 (4172771) 4172771..4173760 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
OPA56_RS20585 (4173757) 4173757..4174578 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
OPA56_RS20590 (4174581) 4174581..4174958 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
OPA56_RS20595 (4174965) 4174965..4175813 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
OPA56_RS20600 (4175840) 4175840..4176124 - 285 WP_001160964.1 CcdB family protein Toxin
OPA56_RS20605 (4176127) 4176127..4176360 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
OPA56_RS20610 (4176446) 4176446..4176925 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
OPA56_RS20615 (4177117) 4177117..4178979 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
OPA56_RS20620 (4178972) 4178972..4179502 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
OPA56_RS20625 (4179610) 4179610..4180941 - 1332 WP_001183209.1 MFS transporter -
OPA56_RS20630 (4181000) 4181000..4181287 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T42705 WP_001160964.1 NZ_AP026788:c4176124-4175840 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T42705 NZ_AP026788:c4176124-4175840 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT42705 WP_000125566.1 NZ_AP026788:c4176360-4176127 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT42705 NZ_AP026788:c4176360-4176127 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References