Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3724937..3725555 Replicon chromosome
Accession NZ_AP026788
Organism Escherichia coli strain IPCEC42

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag OPA56_RS18450 Protein ID WP_001291435.1
Coordinates 3725337..3725555 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag OPA56_RS18445 Protein ID WP_000344800.1
Coordinates 3724937..3725311 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPA56_RS18435 (3720026) 3720026..3721219 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
OPA56_RS18440 (3721242) 3721242..3724391 + 3150 WP_001132478.1 efflux RND transporter permease AcrB -
OPA56_RS18445 (3724937) 3724937..3725311 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
OPA56_RS18450 (3725337) 3725337..3725555 + 219 WP_001291435.1 HHA domain-containing protein Toxin
OPA56_RS18455 (3725729) 3725729..3726280 + 552 WP_000102539.1 maltose O-acetyltransferase -
OPA56_RS18460 (3726396) 3726396..3726866 + 471 WP_000136192.1 YlaC family protein -
OPA56_RS18465 (3727030) 3727030..3728580 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
OPA56_RS18470 (3728622) 3728622..3728975 - 354 WP_000878135.1 DUF1428 family protein -
OPA56_RS18480 (3729354) 3729354..3729665 + 312 WP_000409908.1 MGMT family protein -
OPA56_RS18485 (3729696) 3729696..3730268 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42703 WP_001291435.1 NZ_AP026788:3725337-3725555 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T42703 NZ_AP026788:3725337-3725555 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT42703 WP_000344800.1 NZ_AP026788:3724937-3725311 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT42703 NZ_AP026788:3724937-3725311 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References