Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 65328..65929 Replicon plasmid pIPCEC31_2
Accession NZ_AP026785
Organism Escherichia coli strain IPCEC31

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag OO978_RS24310 Protein ID WP_001216045.1
Coordinates 65328..65708 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag OO978_RS24315 Protein ID WP_001190712.1
Coordinates 65708..65929 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO978_RS24280 60429..60548 - 120 WP_071527722.1 ash family protein -
OO978_RS24285 (IPCEC31_46770) 60770..62254 - 1485 WP_032163774.1 hypothetical protein -
OO978_RS24290 (IPCEC31_46780) 62254..63447 - 1194 WP_016231382.1 hypothetical protein -
OO978_RS24295 (IPCEC31_46790) 63533..63985 - 453 WP_016231381.1 hypothetical protein -
OO978_RS24300 (IPCEC31_46800) 64073..65116 - 1044 WP_264824749.1 DUF968 domain-containing protein -
OO978_RS24305 65144..65323 - 180 WP_000113019.1 hypothetical protein -
OO978_RS24310 (IPCEC31_46810) 65328..65708 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
OO978_RS24315 (IPCEC31_46820) 65708..65929 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
OO978_RS24320 (IPCEC31_46830) 66002..66391 - 390 WP_000506726.1 S24 family peptidase -
OO978_RS24325 (IPCEC31_46840) 66515..66766 - 252 WP_001377386.1 DNA polymerase III subunit theta -
OO978_RS24330 67216..67353 + 138 WP_170988307.1 hypothetical protein -
OO978_RS24340 (IPCEC31_46870) 68204..68566 - 363 WP_001261544.1 hypothetical protein -
OO978_RS24345 (IPCEC31_46880) 68563..69495 - 933 WP_000057449.1 hypothetical protein -
OO978_RS24350 (IPCEC31_46890) 69477..69851 - 375 WP_000988658.1 hypothetical protein -
OO978_RS24355 (IPCEC31_46900) 69858..70151 - 294 WP_050153512.1 hypothetical protein -
OO978_RS24360 (IPCEC31_46910) 70330..70563 - 234 WP_000516537.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..97115 97115
- flank IS/Tn - - 67769..68044 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T42684 WP_001216045.1 NZ_AP026785:c65708-65328 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T42684 NZ_AP026785:c65708-65328 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT42684 WP_001190712.1 NZ_AP026785:c65929-65708 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT42684 NZ_AP026785:c65929-65708 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References