Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 98607..99250 Replicon plasmid pIPCEC31_1
Accession NZ_AP026784
Organism Escherichia coli strain IPCEC31

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag OO978_RS23975 Protein ID WP_001034044.1
Coordinates 98607..99023 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag OO978_RS23980 Protein ID WP_001261286.1
Coordinates 99020..99250 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO978_RS23955 (95020) 95020..95295 + 276 WP_000179213.1 IS1-like element transposase -
OO978_RS23960 (95317) 95317..95706 + 390 WP_071999143.1 IS1 family transposase -
OO978_RS23965 (95960) 95960..96982 - 1023 WP_000361402.1 helicase UvrD -
OO978_RS23970 (96967) 96967..98532 - 1566 WP_001128474.1 AAA family ATPase -
OO978_RS23975 (98607) 98607..99023 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
OO978_RS23980 (99020) 99020..99250 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
OO978_RS23985 (99810) 99810..100028 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
OO978_RS23990 (100030) 100030..100335 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
OO978_RS23995 (100336) 100336..101142 + 807 WP_000016970.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / aadA5 / qacE / sul1 / mph(A) / sitABCD iucA / iucB / iucC / iucD / iutA 1..102618 102618
- flank IS/Tn - - 95359..95706 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T42683 WP_001034044.1 NZ_AP026784:c99023-98607 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T42683 NZ_AP026784:c99023-98607 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT42683 WP_001261286.1 NZ_AP026784:c99250-99020 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT42683 NZ_AP026784:c99250-99020 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References