Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4322345..4322842 Replicon chromosome
Accession NZ_AP026783
Organism Escherichia coli strain IPCEC31

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO978_RS21115 Protein ID WP_001173343.1
Coordinates 4322345..4322518 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag OO978_RS21120 Protein ID WP_001425728.1
Coordinates 4322546..4322842 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO978_RS21105 (4319015) 4319015..4320472 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
OO978_RS21110 (4320709) 4320709..4322226 + 1518 WP_001295074.1 lysine--tRNA ligase -
OO978_RS21115 (4322345) 4322345..4322518 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO978_RS21120 (4322546) 4322546..4322842 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO978_RS21125 (4323069) 4323069..4323341 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO978_RS21130 (4323353) 4323353..4323583 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
OO978_RS21135 (4323764) 4323764..4325395 + 1632 WP_001216487.1 sensor histidine kinase -
OO978_RS21140 (4325392) 4325392..4326111 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - afaB-I / draA 4287095..4322842 35747


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T42678 WP_001173343.1 NZ_AP026783:c4322518-4322345 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T42678 NZ_AP026783:c4322518-4322345 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT42678 WP_001425728.1 NZ_AP026783:c4322842-4322546 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT42678 NZ_AP026783:c4322842-4322546 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References