Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3517184..3517802 Replicon chromosome
Accession NZ_AP026783
Organism Escherichia coli strain IPCEC31

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag OO978_RS17100 Protein ID WP_001291435.1
Coordinates 3517584..3517802 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag OO978_RS17095 Protein ID WP_000344800.1
Coordinates 3517184..3517558 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO978_RS17085 (3512273) 3512273..3513466 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
OO978_RS17090 (3513489) 3513489..3516638 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
OO978_RS17095 (3517184) 3517184..3517558 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
OO978_RS17100 (3517584) 3517584..3517802 + 219 WP_001291435.1 HHA domain-containing protein Toxin
OO978_RS17105 (3517974) 3517974..3518525 + 552 WP_000102568.1 maltose O-acetyltransferase -
OO978_RS17110 (3518641) 3518641..3519111 + 471 WP_000136192.1 YlaC family protein -
OO978_RS17115 (3519275) 3519275..3520825 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
OO978_RS17120 (3520867) 3520867..3521220 - 354 WP_000878140.1 DUF1428 domain-containing protein -
OO978_RS17130 (3521599) 3521599..3521910 + 312 WP_000409911.1 MGMT family protein -
OO978_RS17135 (3521941) 3521941..3522513 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T42676 WP_001291435.1 NZ_AP026783:3517584-3517802 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T42676 NZ_AP026783:3517584-3517802 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT42676 WP_000344800.1 NZ_AP026783:3517184-3517558 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT42676 NZ_AP026783:3517184-3517558 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References