Detailed information of TA system TA042666

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1850998..1851830
• Replicon: chromosome
• Accession: NZ_AP026783
• Organism: Escherichia coli strain IPCEC31

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0Q2Y5N3
• Locus tag: OO978_RS08865
• Protein ID: WP_001514886.1
• Coordinates: 1850998..1851372 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: OO978_RS08870
• Protein ID: WP_072664811.1
• Coordinates: 1851462..1851830 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO978_RS08830 (1846514)	1846514..1846987	+	474	WP_001105385.1	DNA gyrase inhibitor SbmC	-
OO978_RS08835 (1847186)	1847186..1848244	+	1059	WP_032181141.1	FUSC family protein	-
OO978_RS08840 (1848416)	1848416..1848745	+	330	WP_000450409.1	DUF496 family protein	-
OO978_RS08845 (1848846)	1848846..1849112	-	267	WP_063121129.1	EutP/PduV family microcompartment system protein	-
OO978_RS08850 (1849482)	1849482..1849853	-	372	WP_001295631.1	IS110 family transposase	-
OO978_RS08855 (1850669)	1850669..1850749	-	81	Protein_1734	hypothetical protein	-
OO978_RS08860 (1850849)	1850849..1851001	-	153	Protein_1735	DUF5983 family protein	-
OO978_RS08865 (1850998)	1850998..1851372	-	375	WP_001514886.1	TA system toxin CbtA family protein	Toxin
OO978_RS08870 (1851462)	1851462..1851830	-	369	WP_072664811.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
OO978_RS08875 (1851993)	1851993..1852214	-	222	WP_000692345.1	DUF987 domain-containing protein	-
OO978_RS08880 (1852277)	1852277..1852753	-	477	WP_001186747.1	RadC family protein	-
OO978_RS08885 (1852769)	1852769..1853242	-	474	WP_000855059.1	antirestriction protein	-
OO978_RS08890 (1853584)	1853584..1854405	-	822	WP_001234565.1	DUF932 domain-containing protein	-
OO978_RS08895 (1854524)	1854524..1854719	-	196	Protein_1742	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13930.91 Da
• Isoelectric Point: 7.2909

>T42666 WP_001514886.1 NZ_AP026783:c1851372-1850998 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T42666 NZ_AP026783:c1851372-1850998 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13564.25 Da
• Isoelectric Point: 5.8786

>AT42666 WP_072664811.1 NZ_AP026783:c1851830-1851462 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT42666 NZ_AP026783:c1851830-1851462 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q2Y5N3