Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 881332..881986 Replicon chromosome
Accession NZ_AP026783
Organism Escherichia coli strain IPCEC31

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag OO978_RS04315 Protein ID WP_000244777.1
Coordinates 881579..881986 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag OO978_RS04310 Protein ID WP_000354046.1
Coordinates 881332..881598 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO978_RS04285 (876620) 876620..877363 + 744 Protein_840 SDR family oxidoreductase -
OO978_RS04290 (877420) 877420..878853 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
OO978_RS04295 (878898) 878898..879209 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
OO978_RS04300 (879373) 879373..880032 + 660 WP_000250274.1 hemolysin III family protein -
OO978_RS04305 (880109) 880109..881089 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
OO978_RS04310 (881332) 881332..881598 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
OO978_RS04315 (881579) 881579..881986 + 408 WP_000244777.1 protein YgfX Toxin
OO978_RS04320 (882026) 882026..882547 - 522 WP_001055874.1 flavodoxin FldB -
OO978_RS04325 (882659) 882659..883555 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
OO978_RS04330 (883580) 883580..884290 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
OO978_RS04335 (884296) 884296..886029 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T42661 WP_000244777.1 NZ_AP026783:881579-881986 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T42661 NZ_AP026783:881579-881986 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT42661 WP_000354046.1 NZ_AP026783:881332-881598 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT42661 NZ_AP026783:881332-881598 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References