Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 316566..317366 Replicon chromosome
Accession NZ_AP026783
Organism Escherichia coli strain IPCEC31

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag OO978_RS01470 Protein ID WP_000342449.1
Coordinates 316839..317366 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag OO978_RS01465 Protein ID WP_001277108.1
Coordinates 316566..316832 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO978_RS01445 (312223) 312223..312891 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
OO978_RS01450 (312884) 312884..313942 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
OO978_RS01455 (314187) 314187..315041 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
OO978_RS01460 (315313) 315313..316416 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
OO978_RS01465 (316566) 316566..316832 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
OO978_RS01470 (316839) 316839..317366 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
OO978_RS01475 (317363) 317363..317746 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
OO978_RS01480 (318170) 318170..319279 + 1110 WP_122658362.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
OO978_RS01485 (319327) 319327..320253 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
OO978_RS01490 (320250) 320250..321527 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
OO978_RS01495 (321524) 321524..322291 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T42658 WP_000342449.1 NZ_AP026783:316839-317366 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T42658 NZ_AP026783:316839-317366 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT42658 WP_001277108.1 NZ_AP026783:316566-316832 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT42658 NZ_AP026783:316566-316832 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References