Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 4313..4832 Replicon plasmid pNUITMVRE1_2
Accession NZ_AP026775
Organism Enterococcus faecium strain NUITM-VRE1

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag OO969_RS15680 Protein ID WP_000588503.1
Coordinates 4575..4832 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag OO969_RS15675 Protein ID WP_000388479.1
Coordinates 4313..4582 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO969_RS15645 (NUITMVRE1_30910) 303..587 - 285 WP_000353844.1 hypothetical protein -
OO969_RS15650 (NUITMVRE1_30920) 953..1177 + 225 WP_000410576.1 hypothetical protein -
OO969_RS15655 (NUITMVRE1_30930) 2300..2980 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
OO969_RS15660 (NUITMVRE1_30940) 3056..3622 + 567 Protein_4 hypothetical protein -
OO969_RS15665 3787..3987 + 201 WP_001033544.1 4-hydroxybenzoate octaprenyltransferase -
OO969_RS15670 (NUITMVRE1_30950) 4003..4182 + 180 WP_001120991.1 hypothetical protein -
OO969_RS15675 (NUITMVRE1_30960) 4313..4582 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
OO969_RS15680 (NUITMVRE1_30970) 4575..4832 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
OO969_RS15685 (NUITMVRE1_30980) 5470..6078 + 609 WP_224752223.1 hypothetical protein -
OO969_RS15690 (NUITMVRE1_30990) 6212..7531 + 1320 WP_000202380.1 IS1380-like element IS1678 family transposase -
OO969_RS15695 (NUITMVRE1_31000) 7744..7977 + 234 WP_002296232.1 hypothetical protein -
OO969_RS15700 (NUITMVRE1_31010) 8142..8756 - 615 WP_001261742.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX - 1..28491 28491
- inside IScluster/Tn - - 2300..7531 5231


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T42654 WP_000588503.1 NZ_AP026775:4575-4832 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T42654 NZ_AP026775:4575-4832 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT42654 WP_000388479.1 NZ_AP026775:4313-4582 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT42654 NZ_AP026775:4313-4582 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References