Detailed information of TA system TA042653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 224091..224708
• Replicon: plasmid pNUITMVRE1_1
• Accession: NZ_AP026774
• Organism: Enterococcus faecium strain NUITM-VRE1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: OO969_RS15560
• Protein ID: WP_224451579.1
• Coordinates: 224556..224708 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: OO969_RS15555
• Protein ID: WP_002305052.1
• Coordinates: 224091..224522 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO969_RS15540 (NUITMVRE1_30730)	219586..221532	+	1947	WP_002303302.1	sucrose-specific PTS transporter subunit IIBC	-
OO969_RS15545 (NUITMVRE1_30740)	221796..222668	+	873	WP_002292418.1	ROK family protein	-
OO969_RS15550 (NUITMVRE1_30750)	222964..223917	+	954	WP_101699768.1	IS30 family transposase	-
OO969_RS15555 (NUITMVRE1_30760)	224091..224522	-	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
OO969_RS15560 (NUITMVRE1_30770)	224556..224708	-	153	WP_224451579.1	type II toxin-antitoxin system HicA family toxin	Toxin
OO969_RS15565	224802..224981	-	180	Protein_250	hypothetical protein	-
OO969_RS15570	224997..225197	-	201	WP_002324500.1	hypothetical protein	-
OO969_RS15575 (NUITMVRE1_30780)	225362..226057	-	696	WP_002305050.1	hypothetical protein	-
OO969_RS15580 (NUITMVRE1_30790)	226050..226487	-	438	WP_002305049.1	hypothetical protein	-
OO969_RS15585 (NUITMVRE1_30800)	226503..226754	-	252	WP_002287517.1	hypothetical protein	-
OO969_RS15590 (NUITMVRE1_30810)	226754..226960	-	207	WP_002324499.1	hypothetical protein	-
OO969_RS15595	227408..227608	-	201	WP_227005125.1	hypothetical protein	-
OO969_RS15600	227800..227944	+	145	Protein_257	putative holin-like toxin	-
OO969_RS15605 (NUITMVRE1_30820)	228103..228360	-	258	WP_002295625.1	hypothetical protein	-
OO969_RS15610 (NUITMVRE1_30830)	228382..228867	-	486	Protein_259	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..235911	235911
	-	flank	IS/Tn	-	-	222964..223917	953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5815.65 Da
• Isoelectric Point: 10.3560

>T42653 WP_224451579.1 NZ_AP026774:c224708-224556 [Enterococcus faecium]
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK

Nucleotide

• Length: 153 bp

>T42653 NZ_AP026774:c224708-224556 [Enterococcus faecium]
ATTGAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATTTCATCATCATCTTTATAAGGATGGTGTTAGAATCACTGT
TCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCGAAAAATTTTAAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT42653 WP_002305052.1 NZ_AP026774:c224522-224091 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT42653 NZ_AP026774:c224522-224091 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGCTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8