Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 72248..72767 Replicon plasmid pNUITMVRE1_1
Accession NZ_AP026774
Organism Enterococcus faecium strain NUITM-VRE1

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag OO969_RS14730 Protein ID WP_000588503.1
Coordinates 72510..72767 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag OO969_RS14725 Protein ID WP_000388479.1
Coordinates 72248..72517 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO969_RS14715 (NUITMVRE1_28990) 68480..70360 + 1881 WP_002337940.1 beta-glucoside-specific PTS transporter subunit IIABC -
OO969_RS14720 (NUITMVRE1_29000) 70376..71797 + 1422 WP_010861609.1 family 1 glycosylhydrolase -
OO969_RS14725 (NUITMVRE1_29010) 72248..72517 + 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
OO969_RS14730 (NUITMVRE1_29020) 72510..72767 + 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
OO969_RS14735 (NUITMVRE1_29030) 72947..73327 + 381 WP_264879946.1 adenylate/guanylate cyclase domain-containing protein -
OO969_RS14740 (NUITMVRE1_29040) 73365..74045 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
OO969_RS14745 (NUITMVRE1_29050) 74087..74776 + 690 WP_264879947.1 IS6-like element IS1216 family transposase -
OO969_RS14750 (NUITMVRE1_29060) 75056..75478 + 423 WP_002303116.1 hypothetical protein -
OO969_RS14755 (NUITMVRE1_29070) 75488..75691 + 204 WP_002299573.1 hypothetical protein -
OO969_RS14760 (NUITMVRE1_29080) 75902..76486 + 585 WP_002299575.1 recombinase family protein -
OO969_RS14765 (NUITMVRE1_29090) 76675..77355 - 681 WP_010861579.1 IS6-like element IS1216 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..235911 235911


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T42652 WP_000588503.1 NZ_AP026774:72510-72767 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T42652 NZ_AP026774:72510-72767 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCTCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAGTGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT42652 WP_000388479.1 NZ_AP026774:72248-72517 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT42652 NZ_AP026774:72248-72517 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References