Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 56126..57263 Replicon plasmid pELF_mdr
Accession NZ_AP026773
Organism Enterococcus faecium strain NUITM-VRE1

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag OO969_RS13815 Protein ID WP_001284311.1
Coordinates 56400..57263 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag OO969_RS13810 Protein ID WP_000301765.1
Coordinates 56126..56398 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO969_RS13790 (NUITMVRE1_27040) 51697..52272 - 576 WP_001226076.1 recombinase family protein -
OO969_RS13795 (NUITMVRE1_27050) 52418..55384 + 2967 WP_000684987.1 Tn3 family transposase -
OO969_RS13800 (NUITMVRE1_27060) 55443..55802 + 360 Protein_60 chromosome partitioning protein ParA -
OO969_RS13805 (NUITMVRE1_27070) 55894..56109 + 216 WP_001835296.1 peptide-binding protein -
OO969_RS13810 (NUITMVRE1_27080) 56126..56398 + 273 WP_000301765.1 antitoxin Antitoxin
OO969_RS13815 (NUITMVRE1_27090) 56400..57263 + 864 WP_001284311.1 zeta toxin family protein Toxin
OO969_RS13820 (NUITMVRE1_27100) 57437..58846 - 1410 WP_264879932.1 ISL3 family transposase -
OO969_RS13825 (NUITMVRE1_27110) 58999..59418 - 420 WP_016922374.1 fosfomycin resistance bacillithiol transferase FosB3 -
OO969_RS13830 (NUITMVRE1_27130) 59955..60692 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
OO969_RS13835 60817..60900 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
OO969_RS13840 (NUITMVRE1_27140) 61684..62001 + 318 WP_002300567.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid fexA / poxtA / Cfr(D) / erm(B) / VanHAX / fosB3 - 1..138954 138954


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T42650 WP_001284311.1 NZ_AP026773:56400-57263 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T42650 NZ_AP026773:56400-57263 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACCCCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT42650 WP_000301765.1 NZ_AP026773:56126-56398 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT42650 NZ_AP026773:56126-56398 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References