Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 482115..482686 Replicon chromosome
Accession NZ_AP026772
Organism Enterococcus faecium strain NUITM-VRE1

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag OO969_RS02275 Protein ID WP_002286801.1
Coordinates 482345..482686 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag OO969_RS02270 Protein ID WP_002323011.1
Coordinates 482115..482345 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO969_RS02245 (NUITMVRE1_04530) 477248..478579 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
OO969_RS02250 (NUITMVRE1_04540) 478601..479227 + 627 WP_002286816.1 cysteine hydrolase -
OO969_RS02255 (NUITMVRE1_04550) 479410..479991 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
OO969_RS02260 480723..481285 + 563 Protein_448 SOS response-associated peptidase -
OO969_RS02265 (NUITMVRE1_04580) 481490..481828 - 339 WP_002286804.1 hypothetical protein -
OO969_RS02270 (NUITMVRE1_04590) 482115..482345 + 231 WP_002323011.1 hypothetical protein Antitoxin
OO969_RS02275 (NUITMVRE1_04600) 482345..482686 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OO969_RS02280 (NUITMVRE1_04610) 483536..483721 + 186 WP_002304207.1 hypothetical protein -
OO969_RS02285 (NUITMVRE1_04630) 484226..484420 + 195 WP_002297028.1 hypothetical protein -
OO969_RS02290 484611..484860 + 250 Protein_454 transposase -
OO969_RS02295 (NUITMVRE1_04640) 485104..485934 - 831 WP_002286789.1 manganese catalase family protein -
OO969_RS02300 (NUITMVRE1_04650) 486142..487476 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T42649 WP_002286801.1 NZ_AP026772:482345-482686 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T42649 NZ_AP026772:482345-482686 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT42649 WP_002323011.1 NZ_AP026772:482115-482345 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT42649 NZ_AP026772:482115-482345 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References