Detailed information of TA system TA042603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 426865..427400
• Replicon: plasmid pN9T4-1
• Accession: NZ_AP026692
• Organism: Rhodococcus qingshengii strain N9T-4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OQH86_RS32545
• Protein ID: WP_064444974.1
• Coordinates: 426865..427164 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A3G9AJN8
• Locus tag: OQH86_RS32550
• Protein ID: WP_042445813.1
• Coordinates: 427164..427400 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OQH86_RS32520 (RQN9TF_32735)	421883..422296	-	414	WP_003941273.1	hypothetical protein	-
OQH86_RS32525 (RQN9TF_32740)	422355..422690	-	336	WP_241199245.1	hypothetical protein	-
OQH86_RS32530 (RQN9TF_32745)	422989..424059	+	1071	WP_125053520.1	PDDEXK nuclease domain-containing protein	-
OQH86_RS32535 (RQN9TF_32750)	424189..424986	-	798	WP_125053522.1	hypothetical protein	-
OQH86_RS32540 (RQN9TF_32755)	424986..426836	-	1851	WP_125053524.1	hypothetical protein	-
OQH86_RS32545 (RQN9TF_32760)	426865..427164	-	300	WP_064444974.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OQH86_RS32550 (RQN9TF_32765)	427164..427400	-	237	WP_042445813.1	YlcI/YnfO family protein	Antitoxin
OQH86_RS32555	427773..428513	-	741	WP_082920770.1	TetR/AcrR family transcriptional regulator	-
OQH86_RS32560 (RQN9TF_32775)	428510..429478	-	969	WP_064444976.1	DUF2637 domain-containing protein	-
OQH86_RS32565 (RQN9TF_32780)	430109..430303	-	195	WP_232354273.1	hypothetical protein	-
OQH86_RS32570 (RQN9TF_32785)	430420..431202	-	783	WP_029256883.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..565206	565206

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10440.96 Da
• Isoelectric Point: 4.4988

>T42603 WP_064444974.1 NZ_AP026692:c427164-426865 [Rhodococcus qingshengii]
MRPIHEADLDTVRPVLVLTRQVVRPHLGTVTVAPITDEIRGLSTEVSVSAANGLSADAVVSCDHITTIPVSALGNVIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T42603 NZ_AP026692:c427164-426865 [Rhodococcus qingshengii]
ATGCGACCAATTCACGAAGCCGACCTCGACACAGTCCGCCCGGTCCTCGTACTCACCCGACAGGTGGTCCGTCCACACCT
CGGGACCGTCACGGTAGCGCCCATCACCGACGAGATCCGAGGACTGTCCACTGAGGTGTCCGTCAGTGCCGCGAACGGAC
TATCCGCCGACGCGGTGGTCAGTTGCGATCACATCACCACCATTCCCGTCAGTGCACTCGGCAACGTCATCGGTTATCTT
CTGCCAGCACAAGAAGAATCACTGTCCATAGCCGTGCGCGCCGCATTCGATCTCGAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8633.62 Da
• Isoelectric Point: 6.2181

>AT42603 WP_042445813.1 NZ_AP026692:c427400-427164 [Rhodococcus qingshengii]
MSTQIAVRLPDEIVAFLDDEIREQRATSRASVVLRALERERRRQIAARDAEILANATSEHDLSSLAHHAANPQSTGLS

Nucleotide

• Length: 237 bp

>AT42603 NZ_AP026692:c427400-427164 [Rhodococcus qingshengii]
ATGAGCACGCAGATCGCGGTACGACTACCGGACGAGATCGTGGCATTTCTCGACGACGAAATCAGAGAACAGCGCGCCAC
CTCGCGAGCCTCGGTAGTGTTGCGCGCTCTCGAACGCGAGCGTCGCCGACAGATCGCCGCACGTGACGCGGAGATTCTCG
CGAACGCCACATCCGAACATGACCTGAGTTCTCTCGCTCACCACGCCGCCAATCCGCAGTCAACAGGCCTGAGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G9AJN8