Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 399655..400220 Replicon plasmid pN9T4-1
Accession NZ_AP026692
Organism Rhodococcus qingshengii strain N9T-4

Toxin (Protein)


Gene name mazF Uniprot ID C3JNM9
Locus tag OQH86_RS32405 Protein ID WP_003943396.1
Coordinates 399655..400011 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID C3JNM8
Locus tag OQH86_RS32410 Protein ID WP_003943374.1
Coordinates 400008..400220 (-) Length 71 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH86_RS32380 395181..395285 + 105 Protein_412 IS481 family transposase -
OQH86_RS32385 (RQN9TF_32600) 396022..396453 + 432 WP_125053505.1 hypothetical protein -
OQH86_RS32390 397052..397256 + 205 Protein_414 IS110 family transposase -
OQH86_RS32395 (RQN9TF_32605) 397533..398045 + 513 WP_064442835.1 hypothetical protein -
OQH86_RS32400 (RQN9TF_32610) 399068..399457 + 390 WP_007728194.1 helix-turn-helix transcriptional regulator -
OQH86_RS32405 (RQN9TF_32615) 399655..400011 - 357 WP_003943396.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OQH86_RS32410 (RQN9TF_32620) 400008..400220 - 213 WP_003943374.1 ribbon-helix-helix domain-containing protein Antitoxin
OQH86_RS32415 (RQN9TF_32630) 401524..402558 + 1035 WP_047269670.1 diiron oxygenase -
OQH86_RS32420 (RQN9TF_32635) 402769..403467 - 699 WP_125053503.1 IS6 family transposase -
OQH86_RS32425 (RQN9TF_32640) 403662..404876 + 1215 WP_259349443.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..565206 565206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-117)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-39)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12844.58 Da        Isoelectric Point: 6.8817

>T42602 WP_003943396.1 NZ_AP026692:c400011-399655 [Rhodococcus qingshengii]
MIRRAHIHWVDLDPAQGSEAAKRRPAVVVSNDRANATAENLGRGVITVVPLTTNTAHVFPFQVIVESDESGLARASKAQA
EQVRSVSVRRLDPEPVGKVGMETFAALEDALRLHLDLQ

Download         Length: 357 bp

>T42602 NZ_AP026692:c400011-399655 [Rhodococcus qingshengii]
ATGATTCGCCGCGCGCACATTCACTGGGTGGATCTCGATCCGGCGCAGGGCAGTGAAGCGGCGAAACGACGACCGGCGGT
GGTGGTGAGCAACGACCGGGCCAACGCTACTGCAGAAAATCTCGGCAGGGGCGTGATCACCGTGGTTCCGTTGACTACCA
ATACCGCGCACGTGTTTCCGTTTCAGGTGATTGTCGAGTCCGACGAGTCAGGTTTGGCGCGAGCGTCGAAAGCGCAAGCG
GAGCAAGTGCGTTCGGTATCGGTTCGGCGTCTGGATCCTGAACCTGTCGGCAAGGTCGGGATGGAGACTTTCGCCGCACT
CGAGGACGCGCTGCGGCTGCACCTCGATCTGCAGTAA

Antitoxin


Download         Length: 71 a.a.        Molecular weight: 7682.43 Da        Isoelectric Point: 4.1824

>AT42602 WP_003943374.1 NZ_AP026692:c400220-400008 [Rhodococcus qingshengii]
MTTKFTISLPDEDAHLLDEYVRDHGLRGRSAGIRAALRLLSTSGLERDYAAAFAEGDVDEVWDAVTADTE

Download         Length: 213 bp

>AT42602 NZ_AP026692:c400220-400008 [Rhodococcus qingshengii]
ATGACCACGAAATTTACCATAAGTCTGCCTGACGAGGATGCGCACCTACTCGACGAGTATGTGCGTGATCACGGACTTCG
TGGCCGGTCGGCAGGAATTCGAGCCGCACTCCGCCTACTCTCGACGTCCGGACTCGAACGTGACTACGCTGCAGCGTTTG
CTGAAGGTGACGTAGATGAAGTGTGGGACGCTGTCACGGCAGACACCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2PUJ5

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2PWJ9

References