Detailed information of TA system TA042600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 111207..111725
• Replicon: plasmid pN9T4-1
• Accession: NZ_AP026692
• Organism: Rhodococcus qingshengii strain N9T-4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q6XN60
• Locus tag: OQH86_RS30840
• Protein ID: WP_011133429.1
• Coordinates: 111207..111509 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A8E3P5W3
• Locus tag: OQH86_RS30845
• Protein ID: WP_064444958.1
• Coordinates: 111510..111725 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OQH86_RS30825	108131..108238	-	108	Protein_101	ArsR family transcriptional regulator	-
OQH86_RS30830 (RQN9TF_31030)	108411..109208	-	798	WP_149357431.1	hypothetical protein	-
OQH86_RS30835 (RQN9TF_31035)	109208..111178	-	1971	WP_265155216.1	hypothetical protein	-
OQH86_RS30840	111207..111509	-	303	WP_011133429.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OQH86_RS30845 (RQN9TF_31040)	111510..111725	-	216	WP_064444958.1	hypothetical protein	Antitoxin
OQH86_RS30850 (RQN9TF_31045)	111865..112104	-	240	WP_238592762.1	hypothetical protein	-
OQH86_RS30855 (RQN9TF_31050)	112253..113548	-	1296	WP_149357432.1	AAA family ATPase	-
OQH86_RS30860 (RQN9TF_31055)	113619..114806	-	1188	WP_149357433.1	hypothetical protein	-
OQH86_RS30865 (RQN9TF_31060)	114803..114997	-	195	WP_231783855.1	hypothetical protein	-
OQH86_RS30870 (RQN9TF_31065)	114994..116232	-	1239	WP_149357435.1	VWA-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..565206	565206

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10470.01 Da
• Isoelectric Point: 4.7317

>T42600 WP_011133429.1 NZ_AP026692:c111509-111207 [Rhodococcus qingshengii]
VRPIHDAQLGGLCPVLVLTRELVRPHLGTVTVAPIRSEGRGLSTELSLGEGNGVEDGSVVDCDGVTTIPVSALGVNRGFL
LPAQEVLLSRVLRSAFDLES

Nucleotide

• Length: 303 bp

>T42600 NZ_AP026692:c111509-111207 [Rhodococcus qingshengii]
GTGCGGCCGATTCACGACGCCCAGCTTGGAGGTCTTTGTCCGGTGTTGGTTCTGACGAGGGAATTGGTCCGGCCTCATCT
GGGGACGGTCACTGTTGCTCCCATTCGTTCGGAGGGGCGGGGGTTGTCGACGGAACTGAGTTTAGGCGAAGGCAACGGCG
TCGAGGACGGATCGGTTGTCGACTGCGACGGGGTGACGACGATCCCGGTGTCGGCACTCGGGGTGAATCGAGGTTTTCTC
TTGCCGGCTCAGGAAGTGTTGTTGAGCAGGGTTCTGCGATCAGCATTCGATCTCGAATCATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7670.69 Da
• Isoelectric Point: 8.9167

>AT42600 WP_064444958.1 NZ_AP026692:c111725-111510 [Rhodococcus qingshengii]
MSTQIAVRLPEDIVAFLDSEVSGGRAKSRAQVVLRALEREQRRQLAVRDVELLSDGAGSDVNIAAAHGKKN

Nucleotide

• Length: 216 bp

>AT42600 NZ_AP026692:c111725-111510 [Rhodococcus qingshengii]
ATGAGCACGCAGATCGCGGTACGGCTACCCGAGGACATCGTGGCGTTTCTCGACAGCGAGGTCAGCGGTGGACGCGCGAA
AAGCCGTGCCCAAGTGGTGCTTCGAGCGTTGGAGCGTGAGCAGCGTCGGCAGCTGGCGGTCCGGGACGTGGAGCTCTTGT
CTGACGGCGCGGGTAGCGATGTGAATATCGCTGCCGCGCACGGAAAGAAGAACTGA

Structures

Toxin

• AlphaFold DB: Q6XN60