Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2341001..2341572 Replicon chromosome
Accession NZ_AP026666
Organism Enterococcus faecium strain GK961

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag OQH70_RS12070 Protein ID WP_002286801.1
Coordinates 2341001..2341342 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag OQH70_RS12075 Protein ID WP_002323011.1
Coordinates 2341342..2341572 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH70_RS12045 (EfmGK961_23480) 2336212..2337546 - 1335 WP_002303373.1 ABC transporter substrate-binding protein -
OQH70_RS12050 (EfmGK961_23490) 2337753..2338583 + 831 WP_002286789.1 manganese catalase family protein -
OQH70_RS12055 2338827..2339076 - 250 Protein_2332 transposase -
OQH70_RS12060 (EfmGK961_23500) 2339267..2339461 - 195 WP_002297028.1 hypothetical protein -
OQH70_RS12065 (EfmGK961_23520) 2339966..2340151 - 186 WP_002304207.1 hypothetical protein -
OQH70_RS12070 (EfmGK961_23530) 2341001..2341342 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OQH70_RS12075 (EfmGK961_23540) 2341342..2341572 - 231 WP_002323011.1 hypothetical protein Antitoxin
OQH70_RS12080 (EfmGK961_23550) 2341859..2342197 + 339 WP_002286804.1 hypothetical protein -
OQH70_RS12085 2342402..2342964 - 563 Protein_2338 SOS response-associated peptidase -
OQH70_RS12090 (EfmGK961_23580) 2343696..2344277 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
OQH70_RS12095 (EfmGK961_23590) 2344460..2345086 - 627 WP_002286816.1 cysteine hydrolase -
OQH70_RS12100 (EfmGK961_23600) 2345108..2346439 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T42576 WP_002286801.1 NZ_AP026666:c2341342-2341001 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T42576 NZ_AP026666:c2341342-2341001 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT42576 WP_002323011.1 NZ_AP026666:c2341572-2341342 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT42576 NZ_AP026666:c2341572-2341342 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References