Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | Txe-Axe/Txe-RelB |
| Location | 1425294..1425813 | Replicon | chromosome |
| Accession | NZ_AP026666 | ||
| Organism | Enterococcus faecium strain GK961 | ||
Toxin (Protein)
| Gene name | Txe | Uniprot ID | Q848U6 |
| Locus tag | OQH70_RS07370 | Protein ID | WP_000588503.1 |
| Coordinates | 1425556..1425813 (+) | Length | 86 a.a. |
Antitoxin (Protein)
| Gene name | Axe | Uniprot ID | Q83WJ8 |
| Locus tag | OQH70_RS07365 | Protein ID | WP_000388479.1 |
| Coordinates | 1425294..1425563 (+) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OQH70_RS07315 (EfmGK961_14120) | 1420490..1421389 | + | 900 | WP_002303312.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | - |
| OQH70_RS07320 (EfmGK961_14130) | 1421392..1421877 | + | 486 | WP_002303313.1 | single-stranded DNA-binding protein | - |
| OQH70_RS07325 | 1422018..1422122 | - | 105 | WP_008274030.1 | putative holin-like toxin | - |
| OQH70_RS07330 (EfmGK961_14140) | 1422296..1422556 | + | 261 | WP_002295623.1 | hypothetical protein | - |
| OQH70_RS07335 (EfmGK961_14150) | 1422993..1423199 | + | 207 | WP_002347218.1 | hypothetical protein | - |
| OQH70_RS07340 (EfmGK961_14160) | 1423199..1423450 | + | 252 | WP_002296238.1 | hypothetical protein | - |
| OQH70_RS07345 (EfmGK961_14170) | 1423465..1423902 | + | 438 | WP_002300851.1 | hypothetical protein | - |
| OQH70_RS07350 (EfmGK961_14180) | 1423895..1424602 | + | 708 | WP_002303117.1 | hypothetical protein | - |
| OQH70_RS07355 (EfmGK961_14190) | 1424767..1424967 | + | 201 | WP_002347143.1 | hypothetical protein | - |
| OQH70_RS07360 | 1424983..1425163 | + | 181 | Protein_1419 | hypothetical protein | - |
| OQH70_RS07365 (EfmGK961_14200) | 1425294..1425563 | + | 270 | WP_000388479.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| OQH70_RS07370 (EfmGK961_14210) | 1425556..1425813 | + | 258 | WP_000588503.1 | Txe/YoeB family addiction module toxin | Toxin |
| OQH70_RS07375 | 1425893..1426270 | + | 378 | Protein_1422 | helix-turn-helix domain-containing protein | - |
| OQH70_RS07380 (EfmGK961_14220) | 1426449..1426682 | + | 234 | WP_265162002.1 | hypothetical protein | - |
| OQH70_RS07385 | 1426755..1427006 | + | 252 | WP_265162118.1 | hypothetical protein | - |
| OQH70_RS07390 (EfmGK961_14230) | 1426982..1427272 | + | 291 | WP_265162003.1 | hypothetical protein | - |
| OQH70_RS07395 (EfmGK961_14240) | 1427437..1428051 | - | 615 | WP_001261742.1 | recombinase family protein | - |
| OQH70_RS07400 (EfmGK961_14250) | 1428170..1428370 | + | 201 | WP_265162004.1 | hypothetical protein | - |
| OQH70_RS07405 (EfmGK961_14260) | 1428354..1428710 | + | 357 | WP_265162005.1 | hypothetical protein | - |
| OQH70_RS07410 | 1428752..1429057 | - | 306 | Protein_1429 | tyrosine-type recombinase/integrase | - |
| OQH70_RS07415 (EfmGK961_14270) | 1429125..1429709 | + | 585 | WP_002299575.1 | recombinase family protein | - |
| OQH70_RS07420 (EfmGK961_14280) | 1430080..1430727 | + | 648 | Protein_1431 | type VI secretion protein ImpB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | erm(B) / aph(3')-III / tet(M) | ebpC / srtC | 1319388..1468782 | 149394 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 86 a.a. Molecular weight: 10206.53 Da Isoelectric Point: 8.4369
>T42575 WP_000588503.1 NZ_AP026666:1425556-1425813 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY
Download Length: 258 bp
>T42575 NZ_AP026666:1425556-1425813 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10373.51 Da Isoelectric Point: 4.4346
>AT42575 WP_000388479.1 NZ_AP026666:1425294-1425563 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD
Download Length: 270 bp
>AT42575 NZ_AP026666:1425294-1425563 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A828ZXQ8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A828ZWU7 |