Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1401816..1402953 Replicon chromosome
Accession NZ_AP026666
Organism Enterococcus faecium strain GK961

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag OQH70_RS07215 Protein ID WP_001284311.1
Coordinates 1402090..1402953 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag OQH70_RS07210 Protein ID WP_000301765.1
Coordinates 1401816..1402088 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH70_RS07170 (EfmGK961_13860) 1397252..1397569 + 318 WP_002295896.1 AzlD domain-containing protein -
OQH70_RS07175 (EfmGK961_13870) 1397813..1399423 + 1611 WP_002286080.1 polysaccharide biosynthesis protein -
OQH70_RS07180 (EfmGK961_13880) 1399580..1400446 + 867 WP_265145862.1 DegV family protein -
OQH70_RS07185 (EfmGK961_13890) 1400500..1400667 + 168 WP_001015295.1 IS6 family transposase -
OQH70_RS07190 1400640..1400735 - 96 WP_265162107.1 23S rRNA methyltransferase attenuation leader peptide -
OQH70_RS07195 (EfmGK961_13900) 1400721..1401179 + 459 WP_265162108.1 IS6 family transposase -
OQH70_RS07200 (EfmGK961_13910) 1401235..1401492 + 258 Protein_1387 chromosome partitioning protein ParA -
OQH70_RS07205 (EfmGK961_13920) 1401584..1401799 + 216 WP_001835296.1 peptide-binding protein -
OQH70_RS07210 (EfmGK961_13930) 1401816..1402088 + 273 WP_000301765.1 antitoxin Antitoxin
OQH70_RS07215 (EfmGK961_13940) 1402090..1402953 + 864 WP_001284311.1 zeta toxin family protein Toxin
OQH70_RS07220 (EfmGK961_13950) 1403216..1403953 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
OQH70_RS07225 (EfmGK961_13960) 1404703..1405497 - 795 WP_265162109.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
OQH70_RS07230 1405590..1405841 - 252 Protein_1393 GNAT family N-acetyltransferase -
OQH70_RS07235 (EfmGK961_13970) 1406086..1407381 + 1296 WP_265145863.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element erm(B) / aph(3')-III / tet(M) ebpC / srtC 1319388..1468782 149394
- inside IScluster/Tn erm(B) / aph(3')-III - 1400500..1409307 8807


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T42574 WP_001284311.1 NZ_AP026666:1402090-1402953 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T42574 NZ_AP026666:1402090-1402953 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT42574 WP_000301765.1 NZ_AP026666:1401816-1402088 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT42574 NZ_AP026666:1401816-1402088 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References