Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2340114..2340685 Replicon chromosome
Accession NZ_AP026660
Organism Enterococcus faecium strain GK941

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag OQH75_RS11940 Protein ID WP_002286801.1
Coordinates 2340114..2340455 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag OQH75_RS11945 Protein ID WP_002323011.1
Coordinates 2340455..2340685 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH75_RS11915 (EfmGK941_23380) 2335324..2336658 - 1335 WP_002303373.1 ABC transporter substrate-binding protein -
OQH75_RS11920 (EfmGK941_23390) 2336866..2337696 + 831 WP_002286789.1 manganese catalase family protein -
OQH75_RS11925 2337940..2338189 - 250 Protein_2306 transposase -
OQH75_RS11930 (EfmGK941_23400) 2338380..2338574 - 195 WP_002297028.1 hypothetical protein -
OQH75_RS11935 (EfmGK941_23420) 2339079..2339264 - 186 WP_002304207.1 hypothetical protein -
OQH75_RS11940 (EfmGK941_23430) 2340114..2340455 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OQH75_RS11945 (EfmGK941_23440) 2340455..2340685 - 231 WP_002323011.1 hypothetical protein Antitoxin
OQH75_RS11950 (EfmGK941_23450) 2340972..2341310 + 339 WP_002286804.1 hypothetical protein -
OQH75_RS11955 2341515..2342077 - 563 Protein_2312 SOS response-associated peptidase -
OQH75_RS11960 (EfmGK941_23480) 2342809..2343390 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
OQH75_RS11965 (EfmGK941_23490) 2343573..2344199 - 627 WP_002286816.1 cysteine hydrolase -
OQH75_RS11970 (EfmGK941_23500) 2344221..2345552 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T42571 WP_002286801.1 NZ_AP026660:c2340455-2340114 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T42571 NZ_AP026660:c2340455-2340114 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT42571 WP_002323011.1 NZ_AP026660:c2340685-2340455 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT42571 NZ_AP026660:c2340685-2340455 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References