Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1403458..1404595 Replicon chromosome
Accession NZ_AP026660
Organism Enterococcus faecium strain GK941

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag OQH75_RS07125 Protein ID WP_001284311.1
Coordinates 1403732..1404595 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag OQH75_RS07120 Protein ID WP_000301765.1
Coordinates 1403458..1403730 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH75_RS07090 (EfmGK941_14000) 1398892..1399209 + 318 WP_002295896.1 AzlD domain-containing protein -
OQH75_RS07095 (EfmGK941_14010) 1399453..1401063 + 1611 WP_002286080.1 polysaccharide biosynthesis protein -
OQH75_RS07100 (EfmGK941_14020) 1401220..1402086 + 867 WP_265145862.1 DegV family protein -
OQH75_RS07105 (EfmGK941_14030) 1402140..1402820 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
OQH75_RS07110 (EfmGK941_14040) 1402876..1403133 + 258 Protein_1369 chromosome partitioning protein ParA -
OQH75_RS07115 (EfmGK941_14050) 1403225..1403441 + 217 Protein_1370 peptide-binding protein -
OQH75_RS07120 (EfmGK941_14060) 1403458..1403730 + 273 WP_000301765.1 antitoxin Antitoxin
OQH75_RS07125 (EfmGK941_14070) 1403732..1404595 + 864 WP_001284311.1 zeta toxin family protein Toxin
OQH75_RS07130 (EfmGK941_14080) 1404858..1405595 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
OQH75_RS07135 1405720..1405803 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
OQH75_RS07140 (EfmGK941_14090) 1406345..1407139 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
OQH75_RS07145 1407232..1407483 - 252 Protein_1376 GNAT family N-acetyltransferase -
OQH75_RS07150 (EfmGK941_14100) 1407728..1409023 + 1296 WP_265145863.1 ISL3-like element ISEfa11 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element erm(B) / aph(3')-III / tet(M) ebpC / srtC 1321022..1470447 149425
- inside IScluster/Tn erm(B) / aph(3')-III - 1402140..1410949 8809


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T42569 WP_001284311.1 NZ_AP026660:1403732-1404595 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T42569 NZ_AP026660:1403732-1404595 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT42569 WP_000301765.1 NZ_AP026660:1403458-1403730 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT42569 NZ_AP026660:1403458-1403730 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References