Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2342832..2343403 Replicon chromosome
Accession NZ_AP026655
Organism Enterococcus faecium strain GK923

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag OQH82_RS11885 Protein ID WP_002286801.1
Coordinates 2342832..2343173 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag OQH82_RS11890 Protein ID WP_002323011.1
Coordinates 2343173..2343403 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH82_RS11860 (EfmGK923_23270) 2338042..2339376 - 1335 WP_002303373.1 ABC transporter substrate-binding protein -
OQH82_RS11865 (EfmGK923_23280) 2339584..2340414 + 831 WP_002286789.1 manganese catalase family protein -
OQH82_RS11870 2340658..2340907 - 250 Protein_2295 transposase -
OQH82_RS11875 (EfmGK923_23290) 2341098..2341292 - 195 WP_002297028.1 hypothetical protein -
OQH82_RS11880 (EfmGK923_23310) 2341797..2341982 - 186 WP_002304207.1 hypothetical protein -
OQH82_RS11885 (EfmGK923_23320) 2342832..2343173 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OQH82_RS11890 (EfmGK923_23330) 2343173..2343403 - 231 WP_002323011.1 hypothetical protein Antitoxin
OQH82_RS11895 (EfmGK923_23340) 2343690..2344028 + 339 WP_002286804.1 hypothetical protein -
OQH82_RS11900 2344233..2344795 - 563 Protein_2301 SOS response-associated peptidase -
OQH82_RS11905 (EfmGK923_23370) 2345527..2346108 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
OQH82_RS11910 (EfmGK923_23380) 2346291..2346917 - 627 WP_002286816.1 cysteine hydrolase -
OQH82_RS11915 (EfmGK923_23390) 2346939..2348270 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T42566 WP_002286801.1 NZ_AP026655:c2343173-2342832 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T42566 NZ_AP026655:c2343173-2342832 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT42566 WP_002323011.1 NZ_AP026655:c2343403-2343173 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT42566 NZ_AP026655:c2343403-2343173 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References