Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1319..1926 Replicon plasmid pAA94_2
Accession NZ_AP026615
Organism Enterococcus faecium strain AA94

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag OQH77_RS16630 Protein ID WP_002321032.1
Coordinates 1319..1669 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag OQH77_RS16635 Protein ID WP_002287514.1
Coordinates 1663..1926 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH77_RS16625 (EfmAA94_33120) 681..1295 + 615 WP_002318230.1 recombinase family protein -
OQH77_RS16630 (EfmAA94_33130) 1319..1669 - 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OQH77_RS16635 (EfmAA94_33140) 1663..1926 - 264 WP_002287514.1 hypothetical protein Antitoxin
OQH77_RS16640 (EfmAA94_33150) 2079..2243 + 165 WP_164450253.1 hypothetical protein -
OQH77_RS16645 2281..2960 + 680 WP_265161990.1 IS6 family transposase -
OQH77_RS16650 (EfmAA94_33180) 3017..3253 + 237 Protein_5 aminoglycoside phosphotransferase family protein -
OQH77_RS16655 (EfmAA94_33190) 3237..3629 + 393 WP_025189010.1 GNAT family N-acetyltransferase -
OQH77_RS16660 3630..5071 + 1442 Protein_7 aminoglycoside O-phosphotransferase APH(2'')-Ia -
OQH77_RS16665 (EfmAA94_33240) 5190..6362 - 1173 WP_000195429.1 IS256-like element IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..63653 63653


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T42564 WP_002321032.1 NZ_AP026615:c1669-1319 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T42564 NZ_AP026615:c1669-1319 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT42564 WP_002287514.1 NZ_AP026615:c1926-1663 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT42564 NZ_AP026615:c1926-1663 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References