Detailed information of TA system TA042563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 289137..289754
• Replicon: plasmid pAA94_1
• Accession: NZ_AP026613
• Organism: Enterococcus faecium strain AA94

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: OQH77_RS15855
• Protein ID: WP_224451579.1
• Coordinates: 289602..289754 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: OQH77_RS15850
• Protein ID: WP_002305052.1
• Coordinates: 289137..289568 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OQH77_RS15830 (EfmAA94_31440)	284954..286381	-	1428	WP_002361237.1	glycoside hydrolase family 32 protein	-
OQH77_RS15835 (EfmAA94_31450)	286435..287352	-	918	WP_265161943.1	oligosaccharide MFS transporter	-
OQH77_RS15840 (EfmAA94_31460)	287439..287708	-	270	WP_265161944.1	MFS transporter	-
OQH77_RS15845	288011..288963	+	953	Protein_328	IS30 family transposase	-
OQH77_RS15850 (EfmAA94_31490)	289137..289568	-	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
OQH77_RS15855 (EfmAA94_31500)	289602..289754	-	153	WP_224451579.1	type II toxin-antitoxin system HicA family toxin	Toxin
OQH77_RS15860	289848..290027	-	180	Protein_331	hypothetical protein	-
OQH77_RS15865	290043..290243	-	201	WP_002324500.1	hypothetical protein	-
OQH77_RS15870 (EfmAA94_31510)	290408..291103	-	696	WP_002305050.1	hypothetical protein	-
OQH77_RS15875 (EfmAA94_31520)	291096..291533	-	438	WP_002305049.1	hypothetical protein	-
OQH77_RS15880 (EfmAA94_31530)	291549..291800	-	252	WP_002287517.1	hypothetical protein	-
OQH77_RS15885 (EfmAA94_31540)	291800..292006	-	207	WP_002324499.1	hypothetical protein	-
OQH77_RS15890	292454..292678	-	225	WP_074399798.1	hypothetical protein	-
OQH77_RS15895	292846..292990	+	145	Protein_338	putative holin-like toxin	-
OQH77_RS15900 (EfmAA94_31550)	293149..293406	-	258	WP_002295625.1	hypothetical protein	-
OQH77_RS15905 (EfmAA94_31560)	293428..293913	-	486	Protein_340	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	VanHBX / ant(6)-Ia / erm(B)	-	1..321364	321364
	-	flank	IS/Tn	-	-	288379..288963	584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5815.65 Da
• Isoelectric Point: 10.3560

>T42563 WP_224451579.1 NZ_AP026613:c289754-289602 [Enterococcus faecium]
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK

Nucleotide

• Length: 153 bp

>T42563 NZ_AP026613:c289754-289602 [Enterococcus faecium]
ATTGAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATTTCATCATCATCTTTATAAGGATGGTGTTAGAATCACTGT
TCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCGAAAAATTTTAAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT42563 WP_002305052.1 NZ_AP026613:c289568-289137 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT42563 NZ_AP026613:c289568-289137 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGCTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8