Detailed information of TA system TA042560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 486702..487267
• Replicon: chromosome
• Accession: NZ_AP026612
• Organism: Enterococcus faecium strain AA94

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: OQH77_RS02350
• Protein ID: WP_265161849.1
• Coordinates: 486932..487267 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: OQH77_RS02345
• Protein ID: WP_002323011.1
• Coordinates: 486702..486932 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OQH77_RS02320 (EfmAA94_04660)	482183..483511	+	1329	WP_002327434.1	FAD-containing oxidoreductase	-
OQH77_RS02325 (EfmAA94_04670)	483533..484159	+	627	WP_002352507.1	cysteine hydrolase	-
OQH77_RS02330 (EfmAA94_04680)	484342..484923	+	582	WP_002327433.1	TetR/AcrR family transcriptional regulator	-
OQH77_RS02335 (EfmAA94_04690)	485288..485863	+	576	WP_002352505.1	SOS response-associated peptidase family protein	-
OQH77_RS02340 (EfmAA94_04700)	486068..486406	-	339	WP_002306002.1	hypothetical protein	-
OQH77_RS02345 (EfmAA94_04710)	486702..486932	+	231	WP_002323011.1	hypothetical protein	Antitoxin
OQH77_RS02350 (EfmAA94_04720)	486932..487267	+	336	WP_265161849.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
OQH77_RS02355	487608..488065	+	458	Protein_467	transposase	-
OQH77_RS02360 (EfmAA94_04750)	488160..489117	+	958	Protein_468	IS30 family transposase	-
OQH77_RS02365 (EfmAA94_04770)	489123..489833	+	711	Protein_469	IS3-like element IS1485 family transposase	-
OQH77_RS02370	489964..490206	+	243	Protein_470	LPXTG cell wall anchor domain-containing protein	-
OQH77_RS02375 (EfmAA94_04780)	490280..491176	+	897	WP_002352497.1	class C sortase	-
OQH77_RS02380 (EfmAA94_04790)	491357..492031	+	675	WP_002291233.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	455933..672483	216550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12981.28 Da
• Isoelectric Point: 9.9821

>T42560 WP_265161849.1 NZ_AP026612:486932-487267 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIFT

Nucleotide

• Length: 336 bp

>T42560 NZ_AP026612:486932-487267 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATCTTTACTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT42560 WP_002323011.1 NZ_AP026612:486702-486932 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT42560 NZ_AP026612:486702-486932 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A828ZZN9