Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 15441..16578 Replicon plasmid pAA55_4
Accession NZ_AP026609
Organism Enterococcus faecium strain AA55

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag OQH71_RS16045 Protein ID WP_001284311.1
Coordinates 15715..16578 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag OQH71_RS16040 Protein ID WP_000301765.1
Coordinates 15441..15713 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH71_RS16005 (EfmAA55_32270) 11260..11556 + 297 WP_000053907.1 replication control protein PrgN -
OQH71_RS16010 (EfmAA55_32280) 11580..12539 - 960 WP_002287225.1 IS30 family transposase -
OQH71_RS16015 (EfmAA55_32290) 12722..13573 + 852 WP_002287227.1 hypothetical protein -
OQH71_RS16020 (EfmAA55_32300) 13594..13806 + 213 WP_002324171.1 hypothetical protein -
OQH71_RS16025 (EfmAA55_32310) 14239..14919 + 681 WP_002328852.1 IS6-like element IS1216 family transposase -
OQH71_RS16030 14973..15116 + 144 Protein_18 chromosome partitioning protein ParA -
OQH71_RS16035 (EfmAA55_32320) 15208..15424 + 217 Protein_19 peptide-binding protein -
OQH71_RS16040 (EfmAA55_32330) 15441..15713 + 273 WP_000301765.1 antitoxin Antitoxin
OQH71_RS16045 (EfmAA55_32340) 15715..16578 + 864 WP_001284311.1 zeta toxin family protein Toxin
OQH71_RS16050 (EfmAA55_32350) 16841..17578 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
OQH71_RS16055 17703..17786 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
OQH71_RS16060 (EfmAA55_32360) 18328..19122 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
OQH71_RS16065 19215..19466 - 252 Protein_25 GNAT family N-acetyltransferase -
OQH71_RS16070 (EfmAA55_32370) 19585..20226 + 642 WP_265161066.1 IS982 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III - 1..20946 20946
- inside IScluster/Tn erm(B) / aph(3')-III - 2589..20925 18336


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T42558 WP_001284311.1 NZ_AP026609:15715-16578 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T42558 NZ_AP026609:15715-16578 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT42558 WP_000301765.1 NZ_AP026609:15441-15713 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT42558 NZ_AP026609:15441-15713 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References