Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1891..2498 Replicon plasmid pAA55_3
Accession NZ_AP026608
Organism Enterococcus faecium strain AA55

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag OQH71_RS15640 Protein ID WP_002321032.1
Coordinates 2148..2498 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag OQH71_RS15635 Protein ID WP_002287514.1
Coordinates 1891..2154 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH71_RS15625 (EfmAA55_31430) 95..496 + 402 WP_002305759.1 IS200/IS605 family transposase -
OQH71_RS15630 (EfmAA55_31440) 508..1653 + 1146 WP_002305757.1 IS200/IS605 family element RNA-guided endonuclease TnpB -
OQH71_RS15635 (EfmAA55_31450) 1891..2154 + 264 WP_002287514.1 hypothetical protein Antitoxin
OQH71_RS15640 (EfmAA55_31460) 2148..2498 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OQH71_RS15645 (EfmAA55_31470) 2522..3136 - 615 WP_046650770.1 recombinase family protein -
OQH71_RS15650 (EfmAA55_31480) 3450..3872 + 423 WP_002288787.1 hypothetical protein -
OQH71_RS15655 (EfmAA55_31490) 3882..4070 + 189 WP_265161027.1 hypothetical protein -
OQH71_RS15660 (EfmAA55_31500) 4283..4867 + 585 WP_002299575.1 recombinase family protein -
OQH71_RS15665 5036..6361 + 1326 Protein_8 Y-family DNA polymerase -
OQH71_RS15670 (EfmAA55_31530) 6354..6704 + 351 WP_000568378.1 hypothetical protein -
OQH71_RS15675 6701..6880 + 180 Protein_10 hypothetical protein -
OQH71_RS15680 (EfmAA55_31540) 7016..7306 + 291 WP_000026576.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX - 1..50850 50850


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T42557 WP_002321032.1 NZ_AP026608:2148-2498 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T42557 NZ_AP026608:2148-2498 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT42557 WP_002287514.1 NZ_AP026608:1891-2154 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT42557 NZ_AP026608:1891-2154 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References