Detailed information of TA system TA042556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 14666..15185
• Replicon: plasmid pAA55_2
• Accession: NZ_AP026607
• Organism: Enterococcus faecium strain AA55

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: OQH71_RS15410
• Protein ID: WP_000588503.1
• Coordinates: 14666..14923 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: OQH71_RS15415
• Protein ID: WP_000388479.1
• Coordinates: 14916..15185 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OQH71_RS15385 (EfmAA55_30890)	10682..11247	-	566	Protein_15	recombinase family protein	-
OQH71_RS15390 (EfmAA55_30900)	11476..11679	-	204	WP_002299573.1	hypothetical protein	-
OQH71_RS15395 (EfmAA55_30910)	11700..12110	-	411	WP_265160960.1	hypothetical protein	-
OQH71_RS15400 (EfmAA55_30920)	12424..13038	+	615	WP_001261742.1	recombinase family protein	-
OQH71_RS15405 (EfmAA55_30930)	13203..14207	-	1005	WP_000455809.1	hypothetical protein	-
OQH71_RS15410 (EfmAA55_30940)	14666..14923	-	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
OQH71_RS15415 (EfmAA55_30950)	14916..15185	-	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
OQH71_RS15420 (EfmAA55_30960)	15316..15495	-	180	WP_001120991.1	hypothetical protein	-
OQH71_RS15425	15511..15711	-	201	WP_265160963.1	hypothetical protein	-
OQH71_RS15430	16086..17352	+	1267	Protein_24	IS110 family transposase	-
OQH71_RS15435 (EfmAA55_31010)	17641..18276	-	636	WP_021414960.1	hypothetical protein	-
OQH71_RS15440 (EfmAA55_31020)	18258..18674	-	417	WP_002305764.1	hypothetical protein	-
OQH71_RS15445 (EfmAA55_31030)	18690..18941	-	252	WP_002305766.1	hypothetical protein	-
OQH71_RS15450 (EfmAA55_31040)	18941..19147	-	207	WP_002296239.1	hypothetical protein	-
OQH71_RS15455 (EfmAA55_31050)	19592..19852	-	261	WP_002321303.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56468	56468
	-	flank	IS/Tn	-	-	10682..11128	446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T42556 WP_000588503.1 NZ_AP026607:c14923-14666 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T42556 NZ_AP026607:c14923-14666 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT42556 WP_000388479.1 NZ_AP026607:c15185-14916 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT42556 NZ_AP026607:c15185-14916 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7