Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 477753..478324 Replicon chromosome
Accession NZ_AP026604
Organism Enterococcus faecium strain AA55

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag OQH71_RS02270 Protein ID WP_002286801.1
Coordinates 477983..478324 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag OQH71_RS02265 Protein ID WP_002323011.1
Coordinates 477753..477983 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH71_RS02240 (EfmAA55_04630) 472885..474216 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
OQH71_RS02245 (EfmAA55_04640) 474238..474864 + 627 WP_002286816.1 cysteine hydrolase -
OQH71_RS02250 (EfmAA55_04650) 475047..475628 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
OQH71_RS02255 476361..476923 + 563 Protein_448 SOS response-associated peptidase -
OQH71_RS02260 (EfmAA55_04680) 477128..477466 - 339 WP_002286804.1 hypothetical protein -
OQH71_RS02265 (EfmAA55_04690) 477753..477983 + 231 WP_002323011.1 hypothetical protein Antitoxin
OQH71_RS02270 (EfmAA55_04700) 477983..478324 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
OQH71_RS02275 (EfmAA55_04710) 479174..479359 + 186 WP_002304207.1 hypothetical protein -
OQH71_RS02280 (EfmAA55_04730) 479864..480058 + 195 WP_002297028.1 hypothetical protein -
OQH71_RS02285 480249..480498 + 250 Protein_454 transposase -
OQH71_RS02290 (EfmAA55_04740) 480742..481572 - 831 WP_002286789.1 manganese catalase family protein -
OQH71_RS02295 (EfmAA55_04750) 481780..483114 + 1335 WP_002303373.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T42555 WP_002286801.1 NZ_AP026604:477983-478324 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T42555 NZ_AP026604:477983-478324 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT42555 WP_002323011.1 NZ_AP026604:477753-477983 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT42555 NZ_AP026604:477753-477983 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References