Detailed information of TA system TA042551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-Axe/Txe-RelB
• Location: 15937..16456
• Replicon: plasmid pJHP9_3
• Accession: NZ_AP026570
• Organism: Enterococcus faecium strain JHP9

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: Q848U6
• Locus tag: OQH78_RS16865
• Protein ID: WP_000588503.1
• Coordinates: 16199..16456 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: Axe
• Uniprot ID: Q83WJ8
• Locus tag: OQH78_RS16860
• Protein ID: WP_000388479.1
• Coordinates: 15937..16206 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OQH78_RS16820 (EfmJHP9_33910)	11232..11912	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
OQH78_RS16825 (EfmJHP9_33920)	11974..12807	+	834	WP_265169137.1	replication initiation factor domain-containing protein	-
OQH78_RS16830	13007..13189	+	183	Protein_15	hypothetical protein	-
OQH78_RS16835 (EfmJHP9_33930)	13770..13973	+	204	Protein_16	replication protein	-
OQH78_RS16840 (EfmJHP9_33940)	14028..14708	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
OQH78_RS16845 (EfmJHP9_33950)	14764..15246	+	483	Protein_18	hypothetical protein	-
OQH78_RS16850	15411..15611	+	201	WP_001033544.1	4-hydroxybenzoate octaprenyltransferase	-
OQH78_RS16855 (EfmJHP9_33960)	15627..15806	+	180	WP_001120991.1	hypothetical protein	-
OQH78_RS16860 (EfmJHP9_33970)	15937..16206	+	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
OQH78_RS16865 (EfmJHP9_33980)	16199..16456	+	258	WP_000588503.1	Txe/YoeB family addiction module toxin	Toxin
OQH78_RS16870 (EfmJHP9_33990)	17082..17711	+	630	WP_265169138.1	HEPN domain-containing protein	-
OQH78_RS16875 (EfmJHP9_34000)	17672..17914	+	243	WP_265169140.1	hypothetical protein	-
OQH78_RS16880 (EfmJHP9_34010)	18195..19490	-	1296	WP_002296623.1	ISL3 family transposase	-
OQH78_RS16885 (EfmJHP9_34020)	19596..20204	-	609	WP_265169141.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / ant(6)-Ia / aph(3')-III / VanHAX	-	1..45504	45504
	-	inside	IScluster/Tn	-	-	11232..19490	8258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10206.53 Da
• Isoelectric Point: 8.4369

>T42551 WP_000588503.1 NZ_AP026570:16199-16456 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Nucleotide

• Length: 258 bp

>T42551 NZ_AP026570:16199-16456 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10373.51 Da
• Isoelectric Point: 4.4346

>AT42551 WP_000388479.1 NZ_AP026570:15937-16206 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Nucleotide

• Length: 270 bp

>AT42551 NZ_AP026570:15937-16206 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A828ZXQ8

Antitoxin

• AlphaFold DB: A0A828ZWU7